GB1568869A – Process of producing antibacterial and pesticidal solutions from water hyacinth plant
– Google Patents
GB1568869A – Process of producing antibacterial and pesticidal solutions from water hyacinth plant
– Google Patents
Process of producing antibacterial and pesticidal solutions from water hyacinth plant
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Publication number
GB1568869A
GB1568869A
GB41505/77A
GB4150577A
GB1568869A
GB 1568869 A
GB1568869 A
GB 1568869A
GB 41505/77 A
GB41505/77 A
GB 41505/77A
GB 4150577 A
GB4150577 A
GB 4150577A
GB 1568869 A
GB1568869 A
GB 1568869A
Authority
GB
United Kingdom
Prior art keywords
water
extract
water hyacinth
hyacinth
petioles
Prior art date
1977-07-06
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB41505/77A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
1977-07-06
Filing date
1977-10-05
Publication date
1980-06-11
1977-10-05
Application filed by Individual
filed
Critical
Individual
1980-06-11
Publication of GB1568869A
publication
Critical
patent/GB1568869A/en
Status
Expired
legal-status
Critical
Current
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Classifications
A—HUMAN NECESSITIES
A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
A61K36/06—Fungi, e.g. yeasts
A61K36/062—Ascomycota
A61K36/064—Saccharomycetales, e.g. baker’s yeast
A—HUMAN NECESSITIES
A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
A61K36/18—Magnoliophyta (angiosperms)
A61K36/88—Liliopsida (monocotyledons)
Description
(54) PROCESS OF PRODUCING ANTIBACTERIAL AND
PESTICIDAL SOLUTIONS FROM WATER HYACINTH
PLANT
(71) I, GODOFREDO GABATAN MONSOD
JR., a Filipino citizen of 11 Scout Bayorau
St., Quezon City, Philippines, do hereby declare the invention, for which I pray that a patent may be granted to me, and the method by which it is to be performed, to be particularly described in and by the following statement: This invention generally relates to a process of economically extracting valuable components having antibacterial and pesticidal properties from water lily or water hyacinth plants, Eichhornia crassipes, (Mart.) Solms, consisting of alcohol, antibacterial and pesticidal solutions and particularly to a product as antibacterial and pesticidal extracts as produced by the process thereof.
The medicinal extract of the present invention will help solve the high cost of medicine and pesticides and protect the health of the people from diseasecarrying organisms and harmful insects by producing a cheap antibacterial solutions to supply the requirements of specially the poor and under-privileged people.
It is, therefore, the main object of this invention to provide a process of econo mrcally recycling the water content of water hyacinth plants mainly from the leaves and the petioles in order to recover the valuable nutrients consisting of alcohol, and its antibacterial properties and the product whereever produced by the process of the present invention.
The present invention provides a process for producing antibacterial solutions from water hyacinth plants, which process comprises the steps of extracting the water residue from the petioles and leaves of water hyacinth plants by any conventionel extraction machine or mechanical extractor, set tling the recycled water extract in an antiseptic settling tank to remove at least some suspended solids, filtering said water extract, heating it to an elevated temperature below the boiling point of the water extract, preferably 50-80″C, to kill any harmful microorganisms present in the extract, cooling the water extract to room temperature, adding 2-5 grams of Brewers Yeast, 100-300 grams of sugar per litre of the water extract or 600-900 grams of molasses per 32 litres of the water extract, fermenting the mixture until the evolution of carbon dioxide ceases, filtering the fermented water extract through a 200 mesh seive to remove any remaining suspended solids and precipitate and aging the filtrate in a sterilized sealed container for 4-9 months in a dark room to room temperature. The aged solution can then be bottled.
According to the present invention, the alcohol and the antibacterial properties as an extract is produced from the leaves and petioles of water hyacinth plants In a preferred process, the leaves and the petioles of the said plants are cut down from the roots, collected, washed, crushed, put into an expeller machine to separate the water content containing the valuable nutrients and antibacterial properties present in the leaves and petioles, the water extract being passed into settling tanks, the precipitate and the settled solids are discarded and the liquid collected. The collected liquid is heated to a temperature of preferably 50 C-80 C to kill all the harmful microorganisms that are present, cooled to ordinary room temperature, then about 2-5 grams of yeast (Brewer’s Yeast) and 100300 grams of sugar per litre of the extract or 600-900 grams of molasses per 3.5 liter of the water extract are added. The mixture is fermented for 2-3 weeks, with shaking to cause the evolution of carbon dioxide, after which it is filtered through a 200 mesh sieve until all the suspended solids and precipitate are removed. The fermented water extract is then stored in a sterilized sealed container at a cool dark room temperature for proper aging from 4-9 months. The aged extract is now ready for medicinal and other antibacterial preparations.
It is a general object of the present m- vention to provide a workable method of economically processing the water extract from the petioles and leaves of water hyacinth plants which is generally considered as a hazard to fresh water navigation in the United States and other countries such as Laguna Lake in the Philippines and other neighboring countries. This aquatic plant grows so abundantly and rapidly that it becomes one of the biggest single cause of water pollution and recurrent floods every year because the flow of water in the sterams is clogged by the floating and rotting water hyacinth. It is likewise an object of the present invention to provide a simple and rapid process for producing recycled water hyacinth juice extract very cheaply from a raw material source which is otherwise useless and considered a pest under ordinary circumstances. In carrying out the process of invention, the supposedly useless water content from the newly cut leaves and petioles are stored in settling tanks.
The water extract gathered from the petioles and the leaves of the water hyacinth plants are of no value at all and the said water extract are mainly water residues accumulated in the extraction of fiber for pulp from the petioles and protein concentrate from the leaves. The water extract is then recycled in settling tanks to remove the impurities present and passed through the antiseptic filtration tank to remove further the suspended solids and precipitate which were not removed by settling. The analysis of the alcohol extract using the response factor technique, the percentages of the ethanol shows an alcohol content ranging from 7-12 volume percent.
The product obtained by the invention is an alcoholic extract containing valuable components in solutions having antibacterial properties, and was tested for controlling the phatogenic microorganisms on a
Pour Plate Method.
The test by the Pour Plate Method was carried out by the following steps: The nutrient agar was prepared and sterilised.
One (1) ml. of sterile distilled water and a loopful of pathogenic organisms were placed in a Petri dish and heated until it melted. The nutrient agar was poured in the Petri dish containing the pathogenic organisms, said Petri dish was slowly rotated in order to form a homogenous mixture. The mixture was allowed to harden, and was covered with a filter paper moistened with Water Hyacinth Extract plus the control. The Petri dish with the mixture was incubated at above about 35″C, cooled and then the growth of organisms was observed.
First Trial:
1. Water Hyacinth Stem Extract
200 ml.
Alcohol – 8.96% Pathogenic Organisms Used:
Eschirichia coli
Staphylococcus entiritis
Staphylococcus aureus
Positive for antibacterial properties
2. Water Hyacinth Leaft Extract
200 ml.
Alcohol8.3,% Pathogenic Organisms Used:
Eschirichia coli
Staphylococcus entiritis
Staphylococcus aureus
Positive for antibacterial properties
Second Trial:
1. Water Hyacinth Stem Extract
200 ml.
Alcohol – 9.3% Phatogenic Organisms Used:
Eschirichia coli
Staphylococcus entiritis
Staphylococcus aureus
Positive for antibacterial properties
2. Water Hyacinth Leaft Extract
200 ml.
Alcohol – 10.7% Pathogenic Organisms Used: E!schirichia coli
Staphylococcus entiritis
Staphylococcus aureus
Positive for antibacterial properties.
WHAT I gLIM IS: 1. A process of producing antibacterial solutions from water hyacinth plants which comprises the steps of extracting the water residue from the water hyacinth plants’ petioles and leaves, settling the recycled water extract in an antiseptic settling tank to remove at least some suspended solids, filtering said water extract, heating it to an elevated temperature below the boiling point of the water extract to kill any harmful microorganisms present in the extract, cooling the water extract to room temperature, adding 2-5 grams of Yeast (Brewer’s
Yeast), 100-300 grams of sugar per liter of the water extract or 600-900 grams of molasses per 3.5 liter of the water extract, fermenting the mixture until the evolution of carbon dioxide ceases, filtering the fermented water extract through a 200 mesh sieve to remove any remaining suspended solids and precipitate and aging the filtrate in a sterilised sealed container for 4-9 months
**WARNING** end of DESC field may overlap start of CLMS **.
Claims (5)
**WARNING** start of CLMS field may overlap end of DESC **. container at a cool dark room temperature for proper aging from 4-9 months. The aged extract is now ready for medicinal and other antibacterial preparations. It is a general object of the present m- vention to provide a workable method of economically processing the water extract from the petioles and leaves of water hyacinth plants which is generally considered as a hazard to fresh water navigation in the United States and other countries such as Laguna Lake in the Philippines and other neighboring countries. This aquatic plant grows so abundantly and rapidly that it becomes one of the biggest single cause of water pollution and recurrent floods every year because the flow of water in the sterams is clogged by the floating and rotting water hyacinth. It is likewise an object of the present invention to provide a simple and rapid process for producing recycled water hyacinth juice extract very cheaply from a raw material source which is otherwise useless and considered a pest under ordinary circumstances. In carrying out the process of invention, the supposedly useless water content from the newly cut leaves and petioles are stored in settling tanks. The water extract gathered from the petioles and the leaves of the water hyacinth plants are of no value at all and the said water extract are mainly water residues accumulated in the extraction of fiber for pulp from the petioles and protein concentrate from the leaves. The water extract is then recycled in settling tanks to remove the impurities present and passed through the antiseptic filtration tank to remove further the suspended solids and precipitate which were not removed by settling. The analysis of the alcohol extract using the response factor technique, the percentages of the ethanol shows an alcohol content ranging from 7-12 volume percent. The product obtained by the invention is an alcoholic extract containing valuable components in solutions having antibacterial properties, and was tested for controlling the phatogenic microorganisms on a Pour Plate Method. The test by the Pour Plate Method was carried out by the following steps: The nutrient agar was prepared and sterilised. One (1) ml. of sterile distilled water and a loopful of pathogenic organisms were placed in a Petri dish and heated until it melted. The nutrient agar was poured in the Petri dish containing the pathogenic organisms, said Petri dish was slowly rotated in order to form a homogenous mixture. The mixture was allowed to harden, and was covered with a filter paper moistened with Water Hyacinth Extract plus the control. The Petri dish with the mixture was incubated at above about 35″C, cooled and then the growth of organisms was observed. First Trial: 1. Water Hyacinth Stem Extract 200 ml. Alcohol – 8.96% Pathogenic Organisms Used: Eschirichia coli Staphylococcus entiritis Staphylococcus aureus Positive for antibacterial properties 2. Water Hyacinth Leaft Extract 200 ml. Alcohol8.3,% Pathogenic Organisms Used: Eschirichia coli Staphylococcus entiritis Staphylococcus aureus Positive for antibacterial properties Second Trial: 1. Water Hyacinth Stem Extract 200 ml. Alcohol – 9.3% Phatogenic Organisms Used: Eschirichia coli Staphylococcus entiritis Staphylococcus aureus Positive for antibacterial properties 2. Water Hyacinth Leaft Extract 200 ml. Alcohol – 10.7% Pathogenic Organisms Used: E!schirichia coli Staphylococcus entiritis Staphylococcus aureus Positive for antibacterial properties. WHAT I gLIM IS:
1. A process of producing antibacterial solutions from water hyacinth plants which comprises the steps of extracting the water residue from the water hyacinth plants’ petioles and leaves, settling the recycled water extract in an antiseptic settling tank to remove at least some suspended solids, filtering said water extract, heating it to an elevated temperature below the boiling point of the water extract to kill any harmful microorganisms present in the extract, cooling the water extract to room temperature, adding 2-5 grams of Yeast (Brewer’s
Yeast), 100-300 grams of sugar per liter of the water extract or 600-900 grams of molasses per 3.5 liter of the water extract, fermenting the mixture until the evolution of carbon dioxide ceases, filtering the fermented water extract through a 200 mesh sieve to remove any remaining suspended solids and precipitate and aging the filtrate in a sterilised sealed container for 4-9 months
in a dark room at room temperature.
2. A process according to claim 1 wherein the filtered water extract is heated to a temperature of 50-80 C.
3. A process according to claim 1 substantially as herein described.
4. A solution having antibacterial properties whenever produced by the process of any preceding claim.
5. A solution according to claim 4 substantially as herein described.
GB41505/77A
1977-07-06
1977-10-05
Process of producing antibacterial and pesticidal solutions from water hyacinth plant
Expired
GB1568869A
(en)
Applications Claiming Priority (1)
Application Number
Priority Date
Filing Date
Title
PH19959A
PH10662A
(en)
1977-07-06
1977-07-06
Economical process of producing antibacterial and pesticidal solutions from water hyacinth plant
Publications (1)
Publication Number
Publication Date
GB1568869A
true
GB1568869A
(en)
1980-06-11
Family
ID=19933871
Family Applications (1)
Application Number
Title
Priority Date
Filing Date
GB41505/77A
Expired
GB1568869A
(en)
1977-07-06
1977-10-05
Process of producing antibacterial and pesticidal solutions from water hyacinth plant
Country Status (2)
Country
Link
GB
(1)
GB1568869A
(en)
PH
(1)
PH10662A
(en)
1977
1977-07-06
PH
PH19959A
patent/PH10662A/en
unknown
1977-10-05
GB
GB41505/77A
patent/GB1568869A/en
not_active
Expired
Also Published As
Publication number
Publication date
PH10662A
(en)
1977-07-29
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Legal Events
Date
Code
Title
Description
1980-10-29
PS
Patent sealed
1985-06-12
PCNP
Patent ceased through non-payment of renewal fee
