AU1627988A – Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
– Google Patents
AU1627988A – Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
– Google Patents
Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
Info
Publication number
AU1627988A
AU1627988A
AU16279/88A
AU1627988A
AU1627988A
AU 1627988 A
AU1627988 A
AU 1627988A
AU 16279/88 A
AU16279/88 A
AU 16279/88A
AU 1627988 A
AU1627988 A
AU 1627988A
AU 1627988 A
AU1627988 A
AU 1627988A
Authority
AU
Australia
Prior art keywords
oxidized
microorganisms
guanidine hydrochloride
recombinant interleukin
recovering purified
Prior art date
1987-05-11
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU16279/88A
Other versions
AU622860B2
(en
Inventor
John T. Davis
Glenn Dorin
Amy Lim
Flint Smith
Sidney N. Wolfe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NOVARTIS VACCINES AND DIAGNOSTICS Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
1987-05-11
Filing date
1988-03-31
Publication date
1988-12-06
1988-03-31
Application filed by Cetus Corp
filed
Critical
Cetus Corp
1988-12-06
Publication of AU1627988A
publication
Critical
patent/AU1627988A/en
1992-04-30
Application granted
granted
Critical
1992-04-30
Publication of AU622860B2
publication
Critical
patent/AU622860B2/en
1992-11-26
Assigned to CETUS ONCOLOGY CORPORATION
reassignment
CETUS ONCOLOGY CORPORATION
Request to Amend Deed and Register
Assignors: CETUS CORPORATION
1996-12-05
Assigned to CHIRON CORPORATION
reassignment
CHIRON CORPORATION
Alteration of Name(s) in Register under S187
Assignors: CETUS ONCOLOGY CORPORATION
2008-01-31
Assigned to NOVARTIS VACCINES AND DIAGNOSTICS, INC.
reassignment
NOVARTIS VACCINES AND DIAGNOSTICS, INC.
Request to Amend Deed and Register
Assignors: CHIRON CORPORATION
2008-03-31
Anticipated expiration
legal-status
Critical
Status
Expired
legal-status
Critical
Current
Links
Espacenet
Global Dossier
Discuss
Classifications
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07K—PEPTIDES
C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07K—PEPTIDES
C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
C07K14/52—Cytokines; Lymphokines; Interferons
C07K14/54—Interleukins [IL]
C07K14/55—IL-2
Abstract
A process for recovering highly pure, recombinant IL-2 from transformed microorganisms in which the cells are disrupted; impure recombinant IL-2 is isolated in the form of refractile bodies from the disruptate; the impure IL-2 is dissolved and denatured with at least 6 M guanidine hydrochloride containing a reducing agent; the reduced IL-2 is precipitated and resolubilized; the reduced solubilized IL-2 therein is oxidized by a controlled oxidation in which the concentration of guanidine hydrochloride is maintained at least at 6 M; the oxidized IL-2 is refolded by reducing the concentration of guanidine hydrochloride in the solution; and the oxidized, refolded IL-2 is further purified by ion exchange chromatography or hydrophobic interaction chromatography and ion exchange chromatography.
AU16279/88A
1987-05-11
1988-03-31
Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
Expired
AU622860B2
(en)
Applications Claiming Priority (4)
Application Number
Priority Date
Filing Date
Title
US4840887A
1987-05-11
1987-05-11
US048408
1987-05-11
US16714488A
1988-03-25
1988-03-25
US167144
1988-03-25
Publications (2)
Publication Number
Publication Date
AU1627988A
true
AU1627988A
(en)
1988-12-06
AU622860B2
AU622860B2
(en)
1992-04-30
Family
ID=26726091
Family Applications (1)
Application Number
Title
Priority Date
Filing Date
AU16279/88A
Expired
AU622860B2
(en)
1987-05-11
1988-03-31
Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
Country Status (9)
Country
Link
EP
(1)
EP0368857B1
(en)
JP
(1)
JP2955294B2
(en)
AT
(1)
ATE156491T1
(en)
AU
(1)
AU622860B2
(en)
DE
(1)
DE3855986T2
(en)
DK
(1)
DK10189A
(en)
IL
(1)
IL86326A
(en)
NO
(1)
NO176797C
(en)
WO
(1)
WO1988008849A1
(en)
Cited By (2)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
AU619185B2
(en)
*
1987-05-11
1992-01-23
Novartis Vaccines And Diagnostics, Inc.
Improved process for recovering microbially produced il-2
AU658604B2
(en)
*
1991-01-10
1995-04-27
Teijin Limited
Highly sensitive assay of tissue factor and kit therefor
Families Citing this family (12)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
US4929700A
(en)
*
1987-04-16
1990-05-29
Cetus Corporation
Production of purified, biologically active, bacterially produced recombinant human CSF-1
CA1339757C
(en)
1987-04-16
1998-03-17
Robert F. Halenbeck
Production of purified biologically active, bacterially produced recombinant human csf-1
EP0337243A1
(en)
*
1988-04-14
1989-10-18
F. Hoffmann-La Roche Ag
Process for purifying recombinant human interleukin-2
DK580789D0
(en)
*
1989-11-20
1989-11-20
Novo Nordisk As
PROCEDURE FOR PURIFICATION OF POLYPEPTIDES
GB8927546D0
(en)
*
1989-12-06
1990-02-07
Ciba Geigy
Process for the production of biologically active tgf-beta
US5258496A
(en)
*
1990-07-10
1993-11-02
Scios Nova Inc.
Isolation and purification of lung surfactant protein
US5288931A
(en)
*
1991-12-06
1994-02-22
Genentech, Inc.
Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5661001A
(en)
*
1993-08-04
1997-08-26
Ciba-Geigy Corporation
High molecular weight desulphatohirudin
US5663304A
(en)
*
1993-08-20
1997-09-02
Genentech, Inc.
Refolding of misfolded insulin-like growth factor-I
TW517059B
(en)
1994-07-25
2003-01-11
Ciba Geigy Ag
New process for the production of biologically active protein
AU707719B2
(en)
*
1995-07-21
1999-07-15
Amgen, Inc.
Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt
WO2003097669A2
(en)
*
2002-05-17
2003-11-27
Københavns Universitet
Method for purifying denatured proteins having a desired disulfide bond configuration
Family Cites Families (9)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
GR79124B
(en)
*
1982-12-22
1984-10-02
Genentech Inc
US4569790A
(en)
*
1984-03-28
1986-02-11
Cetus Corporation
Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4530787A
(en)
*
1984-03-28
1985-07-23
Cetus Corporation
Controlled oxidation of microbially produced cysteine-containing proteins
US4572798A
(en)
*
1984-12-06
1986-02-25
Cetus Corporation
Method for promoting disulfide bond formation in recombinant proteins
JPH0640832B2
(en)
*
1985-05-10
1994-06-01
味の素株式会社
Method for recovering interleukin 2 polypeptide
US4748234A
(en)
*
1985-06-26
1988-05-31
Cetus Corporation
Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4656255A
(en)
*
1985-09-09
1987-04-07
International Minerals & Chemical Corp.
Protein recovery
US4801686A
(en)
*
1986-09-04
1989-01-31
Immunex Corporation
Purification of recombinant interleukin-1
US4931543A
(en)
*
1987-05-11
1990-06-05
Cetus Corporation
Process for recovering microbially produced interleukin-2
1988
1988-03-31
JP
JP63503562A
patent/JP2955294B2/en
not_active
Expired – Lifetime
1988-03-31
WO
PCT/US1988/001043
patent/WO1988008849A1/en
active
IP Right Grant
1988-03-31
AT
AT88903676T
patent/ATE156491T1/en
not_active
IP Right Cessation
1988-03-31
DE
DE3855986T
patent/DE3855986T2/en
not_active
Expired – Lifetime
1988-03-31
AU
AU16279/88A
patent/AU622860B2/en
not_active
Expired
1988-03-31
EP
EP88903676A
patent/EP0368857B1/en
not_active
Expired – Lifetime
1988-05-10
IL
IL8632688A
patent/IL86326A/en
not_active
IP Right Cessation
1988-12-21
NO
NO885678A
patent/NO176797C/en
not_active
IP Right Cessation
1989
1989-01-11
DK
DK010189A
patent/DK10189A/en
not_active
Application Discontinuation
Cited By (2)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
AU619185B2
(en)
*
1987-05-11
1992-01-23
Novartis Vaccines And Diagnostics, Inc.
Improved process for recovering microbially produced il-2
AU658604B2
(en)
*
1991-01-10
1995-04-27
Teijin Limited
Highly sensitive assay of tissue factor and kit therefor
Also Published As
Publication number
Publication date
NO885678L
(en)
1988-12-21
JP2955294B2
(en)
1999-10-04
NO176797C
(en)
1995-05-31
IL86326A
(en)
1997-02-18
NO885678D0
(en)
1988-12-21
EP0368857A1
(en)
1990-05-23
DK10189D0
(en)
1989-01-11
JPH02503384A
(en)
1990-10-18
DE3855986D1
(en)
1997-09-11
EP0368857B1
(en)
1997-08-06
DK10189A
(en)
1989-01-11
WO1988008849A1
(en)
1988-11-17
ATE156491T1
(en)
1997-08-15
IL86326A0
(en)
1988-11-15
DE3855986T2
(en)
1998-02-05
AU622860B2
(en)
1992-04-30
NO176797B
(en)
1995-02-20
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