AU4597785A – A genetic engineering process for the preparation of hirudins, and means for carrying out this process
– Google Patents
AU4597785A – A genetic engineering process for the preparation of hirudins, and means for carrying out this process
– Google Patents
A genetic engineering process for the preparation of hirudins, and means for carrying out this process
Info
Publication number
AU4597785A
AU4597785A
AU45977/85A
AU4597785A
AU4597785A
AU 4597785 A
AU4597785 A
AU 4597785A
AU 45977/85 A
AU45977/85 A
AU 45977/85A
AU 4597785 A
AU4597785 A
AU 4597785A
AU 4597785 A
AU4597785 A
AU 4597785A
Authority
AU
Australia
Prior art keywords
genetic engineering
hirudins
preparation
carrying
sequences
Prior art date
1984-08-10
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU45977/85A
Other versions
AU584628B2
(en
Inventor
Dieter Brauer
Joachim Engels
Paul Habermann
Eugen Uhlmann
Friedrich Wengenmayer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Pharma AG
Original Assignee
Schering AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
1984-08-10
Filing date
1985-08-09
Publication date
1986-02-13
1985-08-09
Application filed by Schering AG
filed
Critical
Schering AG
1986-02-13
Publication of AU4597785A
publication
Critical
patent/AU4597785A/en
1989-06-01
Application granted
granted
Critical
1989-06-01
Publication of AU584628B2
publication
Critical
patent/AU584628B2/en
2000-11-02
Assigned to AVENTIS PHARMA DEUTSCHLAND GMBH
reassignment
AVENTIS PHARMA DEUTSCHLAND GMBH
Alteration of Name(s) in Register under S187
Assignors: HOECHST AKTIENGESELLSCHAFT
2002-06-20
Assigned to SCHERING AKTIENGESELLSCHAFT
reassignment
SCHERING AKTIENGESELLSCHAFT
Alteration of Name(s) in Register under S187
Assignors: AVENTIS PHARMA DEUTSCHLAND GMBH
2005-08-09
Anticipated expiration
legal-status
Critical
Status
Expired
legal-status
Critical
Current
Links
Espacenet
Global Dossier
Discuss
Classifications
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07K—PEPTIDES
C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C07K14/81—Protease inhibitors
C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C12N15/09—Recombinant DNA-technology
C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N15/70—Vectors or expression systems specially adapted for E. coli
C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
A—HUMAN NECESSITIES
A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
A61K38/00—Medicinal preparations containing peptides
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07K—PEPTIDES
C07K2319/00—Fusion polypeptide
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07K—PEPTIDES
C07K2319/00—Fusion polypeptide
C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07K—PEPTIDES
C07K2319/00—Fusion polypeptide
C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07K—PEPTIDES
C07K2319/00—Fusion polypeptide
C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Abstract
Hirudin can be obtained using a specific DNA sequence in a genetic engineering process. The gene is advantageously synthesised in the form of several fragments which are enzymatically ligated to give two larger part-sequences which are incorporated into hybrid plasmids and amplified in host organisms. The part-sequences are reisolated and then combined to form the complete gene which is incorporated into a hybrid plasmid and the latter is expressed in a host organism.
AU45977/85A
1984-08-10
1985-08-09
A genetic engineering process for the preparation of hirudins, and means for carrying out this process
Expired
AU584628B2
(en)
Applications Claiming Priority (2)
Application Number
Priority Date
Filing Date
Title
DE19843429430
DE3429430A1
(en)
1984-08-10
1984-08-10
GENE TECHNOLOGICAL METHOD FOR PRODUCING HIRUDINE AND MEANS FOR IMPLEMENTING THIS METHOD
DE3429430
1984-08-10
Publications (2)
Publication Number
Publication Date
AU4597785A
true
AU4597785A
(en)
1986-02-13
AU584628B2
AU584628B2
(en)
1989-06-01
Family
ID=6242746
Family Applications (1)
Application Number
Title
Priority Date
Filing Date
AU45977/85A
Expired
AU584628B2
(en)
1984-08-10
1985-08-09
A genetic engineering process for the preparation of hirudins, and means for carrying out this process
Country Status (18)
Country
Link
EP
(1)
EP0171024B1
(en)
JP
(1)
JPH0811073B2
(en)
KR
(1)
KR930009337B1
(en)
AT
(1)
ATE67245T1
(en)
AU
(1)
AU584628B2
(en)
CA
(1)
CA1341124C
(en)
DE
(2)
DE3429430A1
(en)
DK
(1)
DK175337B1
(en)
ES
(1)
ES8605040A1
(en)
FI
(1)
FI91883C
(en)
GR
(1)
GR851962B
(en)
HU
(1)
HU206135B
(en)
IE
(1)
IE58485B1
(en)
IL
(1)
IL76059A
(en)
NO
(1)
NO177434C
(en)
NZ
(1)
NZ213049A
(en)
PT
(1)
PT80930B
(en)
ZA
(1)
ZA856026B
(en)
Cited By (5)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
AU597238B2
(en)
*
1984-12-13
1990-05-31
Gen-Bio-Tec Gesellschaft fur gentechnologische und Biotechnologische Forschung MBH
Dna sequence encoding a hirudin-like protein and process for preparing such protein
US5286714A
(en)
*
1991-12-07
1994-02-15
Hoechst Aktiengesellschaft
Synthetic isohirudins with improved stability
US5322926A
(en)
*
1988-10-21
1994-06-21
Hoechst Aktiengesellschaft
Isohirudins
US5919895A
(en)
*
1990-03-22
1999-07-06
Consortium Fur Elektrochemische Industrie Gmbh
Secretion of hirudin derivatives
US6514730B1
(en)
1991-03-21
2003-02-04
Consortium für elektrochemische Industrie GmbH
Secretion of hirudin derivatives
Families Citing this family (34)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
DE3438296A1
(en)
*
1984-04-18
1985-11-07
Hoechst Ag, 6230 Frankfurt
NEW POLYPEPTIDES WITH A BLOOD-CLOTHING EFFECT, METHOD FOR THE PRODUCTION OR THEIR RECOVERY, THEIR USE AND THE CONTAINERS THEREOF
DE3738541A1
(en)
*
1987-11-13
1989-05-24
Hoechst Ag
METHOD FOR INSULATING AND CLEANING HIRUDINE
FR2593518B1
(en)
*
1985-05-02
1989-09-08
Transgene Sa
VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS
DE3689525D1
(en)
*
1985-07-17
1994-02-24
Hoechst Ag
New polypeptides with an anticoagulant effect, processes for their preparation or extraction, their use and agents containing them.
DE3541856A1
(en)
*
1985-11-27
1987-06-04
Hoechst Ag
EUKARYOTIC FUSION PROTEINS, THEIR PRODUCTION AND USE, AND MEANS FOR CARRYING OUT THE PROCESS
MY101203A
(en)
*
1985-12-12
1991-08-17
Ucp Gen Pharma Ag
Production of thrombin iinhibitors.
GB8530631D0
(en)
1985-12-12
1986-01-22
Ciba Geigy Ag
Thrombin inhibitors
GB2188322A
(en)
*
1986-03-26
1987-09-30
Bayer Ag
Aprotinin and analogues thereof produced by a recombinant host
GB2188933A
(en)
*
1986-04-10
1987-10-14
Bayer Ag
Expression vectors for production of polypeptides, method for enhanced expression of polypeptides, hosts containing the expression vectors, products manufactured thereby
MC1834A1
(en)
*
1986-07-10
1988-06-03
Transgene Sa
DNA FUNCTIONAL BLOCK AND PLASMID ENCODING HIRUDINE, TRANSFORMED YEAST, PROCESS FOR PREPARING HIRUDINE, HIRUDINE OBTAINED AND ITS USE
FR2607516B2
(en)
*
1986-12-01
1989-12-22
Transgene Sa
IMPROVED VECTORS FOR EXPRESSING A HIRUDIN VARIANT IN YEAST, PROCESS AND PRODUCT OBTAINED
FR2607517B2
(en)
*
1986-12-01
1989-12-22
Transgene Sa
VECTORS FOR EXPRESSING HIRUDIN VARIANTS IN YEAST, PROCESS AND PRODUCT OBTAINED
GB2199582A
(en)
*
1987-01-07
1988-07-13
Bayer Ag
Analogues of pancreatic secretory trypsin inhibitor
US5032573A
(en)
*
1987-03-23
1991-07-16
Bayer Aktiengesellschaft
Homologs of aprotinin produced from a recombinant host, process, expression vector and recombinant host therefor and pharmaceutical use thereof
US5192745A
(en)
*
1987-05-21
1993-03-09
Merrell Dow Pharmaceuticals Inc.
Cyclic anticoagulant peptides
US5236898A
(en)
*
1987-05-21
1993-08-17
Merrell Dow Pharmaceuticals Inc.
Cyclic anticoagulant peptides
GB8723661D0
(en)
*
1987-10-08
1987-11-11
British Bio Technology
Synthetic gene
GB8723662D0
(en)
*
1987-10-08
1987-11-11
British Bio Technology
Synthetic gene
US5256559A
(en)
*
1988-03-04
1993-10-26
Biogen, Inc.
Methods and compositions for inhibiting platelet aggregation
FR2628429B1
(en)
*
1988-03-08
1990-12-28
Transgene Sa
HIRUDINE VARIANTS, USES THEREOF AND PROCESSES FOR OBTAINING THEM
DE3819079A1
(en)
*
1988-06-04
1989-12-07
Hoechst Ag
HIRUDINE DERIVATIVES WITH DELAYED EFFECT
US5167960A
(en)
*
1988-08-03
1992-12-01
New England Deaconess Hospital Corporation
Hirudin-coated biocompatible substance
US5112615A
(en)
*
1988-08-03
1992-05-12
New England Deaconess Hospital Corporation
Soluble hirudin conjugates
US5192747A
(en)
*
1989-10-03
1993-03-09
Merrell Dow Pharmaceuticals Inc.
Anticoagulant peptides
GB9010552D0
(en)
*
1990-05-10
1990-07-04
Erba Carlo Spa
Method for the recombinant production of hirudins and novel hirudins
US5972648A
(en)
*
1993-09-28
1999-10-26
Japan Energy Corporation
Hirudin analogs, methods of manufacture thereof and anticoagulant compositions having these as active ingredients
DE4404168A1
(en)
*
1994-02-10
1995-08-17
Hoechst Ag
Hirudin derivatives and process for their preparation
DE19529997C1
(en)
*
1995-08-16
1997-04-10
Hoechst Ag
A method for inactivating carboxypeptidase Y in hirudin-containing culture broths
DE19543737A1
(en)
1995-11-24
1997-05-28
Hoechst Ag
Process for the ultrafiltration of biological matrices containing peptides or proteins
DE19544233A1
(en)
*
1995-11-28
1997-06-05
Hoechst Ag
Process for using the yeast ADH II promoter system for the biotechnological production of heterologous proteins in high yields
DE19607239A1
(en)
1996-02-27
1997-08-28
Behringwerke Ag
Pharmaceutical composition containing hirudin and process for its preparation
DE19944870A1
(en)
*
1999-09-18
2001-03-29
Aventis Pharma Gmbh
Signal sequences for the production of Leu-hirudin via secretion by E. coli in the culture medium
DE10149678A1
(en)
2001-10-09
2009-03-12
Novel Science International Gmbh
New peptide compounds containing upto 6 aminoacid residues, are potent and specific thrombin inhibitors used for treating and diagnosing diseases associated with thrombin
DE10301255A1
(en)
2003-01-15
2004-07-29
Cpi Creative Pharma International Gmbh
Organic compounds with biological action as thrombin inhibitors and their use
Family Cites Families (4)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
DK339781A
(en)
*
1980-08-05
1982-02-06
Searle & Co
SYNTHETIC GEN
DE3342139A1
(en)
*
1983-11-22
1985-05-30
Ciba-Geigy Ag, Basel
DESULFATOHIRUDINE, METHOD FOR THE PRODUCTION THEREOF AND PHARMACEUTICAL AGENTS
WO1985004418A1
(en)
*
1984-03-27
1985-10-10
Transgene S.A.
Vectors for the expression of hirudine, transformed cells and process for the preparation of hirudine
EP0168342B1
(en)
*
1984-06-14
1991-07-03
Ciba-Geigy Ag
Process for the preparation of thrombin inhibitors
1984
1984-08-10
DE
DE19843429430
patent/DE3429430A1/en
not_active
Withdrawn
1985
1985-07-09
IL
IL76059A
patent/IL76059A/en
not_active
IP Right Cessation
1985-07-30
AT
AT85109567T
patent/ATE67245T1/en
not_active
IP Right Cessation
1985-07-30
EP
EP85109567A
patent/EP0171024B1/en
not_active
Expired – Lifetime
1985-07-30
DE
DE8585109567T
patent/DE3584053D1/en
not_active
Expired – Lifetime
1985-08-05
HU
HU852980A
patent/HU206135B/en
unknown
1985-08-08
ES
ES545994A
patent/ES8605040A1/en
not_active
Expired
1985-08-08
NZ
NZ213049A
patent/NZ213049A/en
unknown
1985-08-08
FI
FI853056A
patent/FI91883C/en
not_active
IP Right Cessation
1985-08-09
DK
DK198503645A
patent/DK175337B1/en
not_active
IP Right Cessation
1985-08-09
PT
PT80930A
patent/PT80930B/en
unknown
1985-08-09
IE
IE197385A
patent/IE58485B1/en
not_active
IP Right Cessation
1985-08-09
CA
CA000488468A
patent/CA1341124C/en
not_active
Expired – Fee Related
1985-08-09
AU
AU45977/85A
patent/AU584628B2/en
not_active
Expired
1985-08-09
NO
NO853155A
patent/NO177434C/en
not_active
IP Right Cessation
1985-08-09
ZA
ZA856026A
patent/ZA856026B/en
unknown
1985-08-09
JP
JP60174404A
patent/JPH0811073B2/en
not_active
Expired – Lifetime
1985-08-09
GR
GR851962A
patent/GR851962B/el
unknown
1985-08-10
KR
KR1019850005776A
patent/KR930009337B1/en
not_active
IP Right Cessation
Cited By (7)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
AU597238B2
(en)
*
1984-12-13
1990-05-31
Gen-Bio-Tec Gesellschaft fur gentechnologische und Biotechnologische Forschung MBH
Dna sequence encoding a hirudin-like protein and process for preparing such protein
US5322926A
(en)
*
1988-10-21
1994-06-21
Hoechst Aktiengesellschaft
Isohirudins
US5919895A
(en)
*
1990-03-22
1999-07-06
Consortium Fur Elektrochemische Industrie Gmbh
Secretion of hirudin derivatives
US6514730B1
(en)
1991-03-21
2003-02-04
Consortium für elektrochemische Industrie GmbH
Secretion of hirudin derivatives
US5286714A
(en)
*
1991-12-07
1994-02-15
Hoechst Aktiengesellschaft
Synthetic isohirudins with improved stability
US5316947A
(en)
*
1991-12-07
1994-05-31
Hoechst Aktiengesellschaft
Synthetic isohirudins with improved stability
US5616476A
(en)
*
1991-12-07
1997-04-01
Hoechst Aktiengesellschaft
Synthetic isohirudins with improved stability
Also Published As
Publication number
Publication date
CA1341124C
(en)
2000-10-24
FI853056L
(en)
1986-02-11
JPS6147198A
(en)
1986-03-07
GR851962B
(en)
1985-12-03
FI91883B
(en)
1994-05-13
JPH0811073B2
(en)
1996-02-07
ZA856026B
(en)
1986-03-26
PT80930A
(en)
1985-09-01
IE58485B1
(en)
1993-09-22
DK175337B1
(en)
2004-08-30
FI91883C
(en)
1994-08-25
KR930009337B1
(en)
1993-09-27
IL76059A
(en)
1993-08-18
ATE67245T1
(en)
1991-09-15
DK364585A
(en)
1986-02-11
KR870002257A
(en)
1987-03-30
EP0171024B1
(en)
1991-09-11
PT80930B
(en)
1987-11-11
AU584628B2
(en)
1989-06-01
HUT38968A
(en)
1986-07-28
NZ213049A
(en)
1988-05-30
DK364585D0
(en)
1985-08-09
ES8605040A1
(en)
1986-02-16
FI853056A0
(en)
1985-08-08
ES545994A0
(en)
1986-02-16
NO177434B
(en)
1995-06-06
DE3429430A1
(en)
1986-02-20
IE851973L
(en)
1986-02-10
NO853155L
(en)
1986-02-11
IL76059A0
(en)
1985-12-31
HU206135B
(en)
1992-08-28
EP0171024A1
(en)
1986-02-12
NO177434C
(en)
1995-09-13
DE3584053D1
(en)
1991-10-17
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Legal Events
Date
Code
Title
Description
2000-11-02
PC
Assignment registered
Owner name:
AVENTIS PHARMA DEUTSCHLAND GMBH
Free format text:
FORMER OWNER WAS: HOECHST AKTIENGESELLSCHAFT
2002-06-20
PC
Assignment registered
Owner name:
SCHERING AKTIENGESELLSCHAFT
Free format text:
FORMER OWNER WAS: AVENTIS PHARMA DEUTSCHLAND GMBH
