AU589384B2 – A method of binding microflora
– Google Patents
AU589384B2 – A method of binding microflora
– Google Patents
A method of binding microflora
Info
Publication number
AU589384B2
AU589384B2
AU50972/85A
AU5097285A
AU589384B2
AU 589384 B2
AU589384 B2
AU 589384B2
AU 50972/85 A
AU50972/85 A
AU 50972/85A
AU 5097285 A
AU5097285 A
AU 5097285A
AU 589384 B2
AU589384 B2
AU 589384B2
Authority
AU
Australia
Prior art keywords
bacteria
adhesion
adhesive promoting
promoting protein
animals
Prior art date
1984-11-08
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU50972/85A
Other versions
AU589384C
(en
AU5097285A
(en
Inventor
Patricia Conway
Staffan Kjelleberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemical Dynamics Sweden AB
Original Assignee
Chemical Dynamics Sweden AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
1984-11-08
Filing date
1985-11-05
Publication date
1989-10-12
1985-11-05
Application filed by Chemical Dynamics Sweden AB
filed
Critical
Chemical Dynamics Sweden AB
1986-06-03
Publication of AU5097285A
publication
Critical
patent/AU5097285A/en
1989-10-12
Publication of AU589384B2
publication
Critical
patent/AU589384B2/en
1990-08-30
Application granted
granted
Critical
1990-08-30
Publication of AU589384C
publication
Critical
patent/AU589384C/en
2005-11-05
Anticipated expiration
legal-status
Critical
Status
Ceased
legal-status
Critical
Current
Links
Espacenet
Global Dossier
Discuss
Classifications
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
C12P21/00—Preparation of peptides or proteins
A—HUMAN NECESSITIES
A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
A61K35/66—Microorganisms or materials therefrom
A61K35/74—Bacteria
A—HUMAN NECESSITIES
A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
A61P1/12—Antidiarrhoeals
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
C12N1/20—Bacteria; Culture media therefor
C12N1/205—Bacterial isolates
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 – C12P39/00, by using microorganisms or enzymes
C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 – C12P39/00, by using microorganisms or enzymes by using bacteria
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C – C12Q, RELATING TO MICROORGANISMS
C12R2001/00—Microorganisms ; Processes using microorganisms
C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
C12R2001/225—Lactobacillus
Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/00—Chemistry: molecular biology and microbiology
Y10S435/8215—Microorganisms
Y10S435/822—Microorganisms using bacteria or actinomycetales
Y10S435/853—Lactobacillus
Abstract
PCT No. PCT/SE85/00431 Sec. 371 Date Jul. 8, 1986 Sec. 102(e) Date Jul. 8, 1986 PCT Filed Nov. 5, 1985 PCT Pub. No. WO86/02837 PCT Pub. Date May 22, 1986.The adhesion of non-pathogenic bacteria to the stomach and intestines of humans and animals is increased by the administration of the bacteria in the presence of a protein, designated adhesive promoting protein, which can be produced by cultivating lactic acid bacteria in a medium with the addition of forms of sugar of enhanced concentration. A preparation to increase said adhesion contains non-pathogenic bacteria cultivated in the manner described above, together with adhesive promoting protein.
Description
A method of binding microflora and preparations therefor
The present invention relates to a method for microbial colonisation of the oesophagus, stomach and intestines of animals and humans by utilizing bacteria beneficial to the host organism. The invention also relates to a method strengthening the mechanism for adhesion of desired species of bacteria applied. The invention also relates to prepa¬ rations for achieving the results mentioned above.
The invention will be described and is applicable for both animals and humans. «Animals» here relates to domestic animals such as pigs, calves and poultry, such as chickens, turke ‘s, geese and ducks.
At present the mortality rate amongst the above-mentioned animals is high, and is caused by the establishment and colonisation of pathogenic bacteria in the stomach and in- testines. Pathogenic bacteria out compete the normal bac¬ teria flora, adhere to the wall of the intestines and give rise to symptoms of disease such as diarrhoea, and results in increased mortality. The losses in some herds or flocks are considerable as a result of outbreaks of pathogenic bac- taria as Escherichia ccli, Salmonella typhimurium, Salmo¬ nella sp. , Shi-gella sp. and Clostridium perfringens.
Numerous attempts are currently being made to introduce nonpathogenic bacteria into the gastro intestinal tract in order to prevent or remove colonisation of pathogenic bacteria. Oral administration of different types of ba¬ cillus to humans has been found to reduce the risk of can¬ cer in the large intestine and increase the removal or ousting of intestinal pathogens as well as being of thera¬ peutic value of elderly patients.Equivalent studies of animals and humans have the common factor that bacteria introduced in the gastro intestinal tract in order to
„i„•»_.»___ – _ . -..
improve the state of health and increase survival in the host organism. Coloτιisation of these bacteria is possible provided the bacteria cells are capable of attaching them¬ selves to the epithelium walls of the host organism.
An almost continuous supply of large bacteria cultures is required to achieve the above results, which makes the me¬ thod impractical .
Most studies of the above type have been carried out on animals and show negative results with respect to adhe- sion and colonisation of the specific type of bacteria used. Existing methods have the following problems,which also prevent successful experiments:
1. Administering bacteria strains of non-specific type which do not exhibit selective colonisation or sur- vival.
2. Administering bacterial strains whose adhesion ability has been investigated and determined in in vitro experiments. The limitation here is that the mechanism for adhesion is not defined and speci- fie (lectinmediated) binding cannot be determined.
Some examples illustrate these limitations:
In pigs:
1. Lactobacillus acidophilus from foodstuff was admini¬ stered in the normal manner. 2. Lactobacillus sp. which had been isolated from the animal was administered orally Colonisation was not obtained in either case.
