AU621604B2

AU621604B2 – Antihypertensive hyperimmune milk, production, composition, and use
– Google Patents

AU621604B2 – Antihypertensive hyperimmune milk, production, composition, and use
– Google Patents
Antihypertensive hyperimmune milk, production, composition, and use

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Publication number
AU621604B2

AU621604B2
AU33500/89A
AU3350089A
AU621604B2
AU 621604 B2
AU621604 B2
AU 621604B2
AU 33500/89 A
AU33500/89 A
AU 33500/89A
AU 3350089 A
AU3350089 A
AU 3350089A
AU 621604 B2
AU621604 B2
AU 621604B2
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AU
Australia
Prior art keywords
milk
hypertensive
factor
animal
hypertensive factor
Prior art date
1988-02-26
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AU33500/89A
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AU3350089A
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Inventor
Lee R. Beck
Ralph J. Stolle
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Stolle Milk Biologics Inc

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Stolle Research and Development Corp
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1988-02-26
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1989-02-23
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1992-03-19

1989-02-23
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1989-09-22
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patent/AU3350089A/en

1992-03-19
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1992-03-19
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1998-12-03
Assigned to STOLLE MILK BIOLOGICS, INC.
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STOLLE MILK BIOLOGICS, INC.
Alteration of Name(s) in Register under S187
Assignors: STOLLE RESEARCH & DEVELOPMENT CORPORATION

2009-02-23
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Classifications

A—HUMAN NECESSITIES

A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES

A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF

A23C9/00—Milk preparations; Milk powder or milk powder preparations

A23C9/20—Dietetic milk products not covered by groups A23C9/12 – A23C9/18

A—HUMAN NECESSITIES

A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS’ REQUISITES

A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF

A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form

A24B15/18—Treatment of tobacco products or tobacco substitutes

A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances

A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances

A—HUMAN NECESSITIES

A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE

A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P9/00—Drugs for disorders of the cardiovascular system

A61P9/12—Antihypertensives

C—CHEMISTRY; METALLURGY

C07—ORGANIC CHEMISTRY

C07K—PEPTIDES

C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof

C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans

C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates

C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals

A—HUMAN NECESSITIES

A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE

A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES

A61K38/00—Medicinal preparations containing peptides

C—CHEMISTRY; METALLURGY

C07—ORGANIC CHEMISTRY

C07K—PEPTIDES

C07K2317/00—Immunoglobulins specific features

C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production

C07K2317/11—Immunoglobulins specific features characterized by their source of isolation or production isolated from eggs

Abstract

Hyperimmunized mammals produce milk containing an antihypertensive factor. The milk or the isolated factor can be used to treat hypertension.

Description

Ei3rnlaour ntcw’; Exeutive vice R.oasdcent T~I-1 CMMt~ISIE’3R OF TfEJTr.
33500 8″ OPI DATE 22/09/89 AP INE AOJP DATE 19/10f
P
t INTERN! 6 ‘PLN. ID 4 e 4S89/0/18 ‘cysvf N TREATY (PCT) (51) Internation!ml Patent Classification 4 (11) International Publication Nunber: WO 89/ 07948 A61K 39/12 Al (43) International Publication Date: 8 September 1989 (08.09.89) (21) International Application Number: PCT/US89/00718 (81) Designated States: AU, DK, JP.
(22) International Filing Date: 23 February 1989 (23.02.89) Published With international search report.
(31) Priority Application Number: 161,039 (32) Priority Date: 26 February 1988 (26.02.88) (33) Priority Country: US (71))Applicant: STOLLE RESEARCH AND DEVELOP- MENT CORPORATION [US/US]; 2964 State Route 42, South, Lebanon, OH 45036 (US).
(72) Inventors: BECK, Lee, R. 1670 Liberty Keuter Road, Lebanon, OH 45036 STOLLE, Ralph, J. 2099 Waynesville Road, Lebanon, OH 45036 (US).
(74) Agents: FOX, Samuel, L. et al.; Saidman, Sterne, Kessler Golustein, 1225 Connect-cut Avenue, N.W., Suite 300, Washington, DC 20036 (US).
(54) Title: ANTIHYPERTENSIVE HYPERIMMUNE MILK, PRODUCTION, COMPOSITION, AND USE (57) Abstract The invention relates to a method for inducing the production of a milk anti-hypertensive factor in an animal, to a method for the isolation of said factor from the milk of said animal in a substantia’y pure form, and to the use of said factor to treat hypertension in humans and other animals.
0J 0t r C- L 11_:1 7 ‘WO 89/07948 PCT/US89/00718 1 TITLE OF THE INVENTION ANTIHYPERTENSIVE HYPERIMMUNE MILK, PRODUCTION, COMPOSITION, AND USE CROSS-REFERENCES TO RELATED DOCUMENTS The present application is a continuation-in-part of Application Serial No. 001,848, filed January 9, 1987, which is a division of Application Serial No. 546,162, filed October 27, 1983, now U.S. Patent No. 4,636,384, which is a continuation-in-part of Application Serial No. 384,625, filed June 3, 1982, now abandoned.
BACKGROUND OF THE INVENTION FIELD OF THE INVENTION This invention relates to the production of a biologic composition that reduces blood pressure, and to the use of such compositions in lowering elevated arterial pressure in subjects.
DESCRIPTION OF THE BACKGROUND ART An elevated arterial pressure hypertension, high blood pressure) is probably the most important public health problem in developed countries–being common, asymptomatic, readily detectable, and often leading to lethal complications ‘C i) 11 WO 89/07948 PCT/US89/00718 -14- In a preferred embodiment, hyperimmunization of bovids may be achieved by a single administration of microencapsu- ‘i A WO 89/07948 PCT/US89/00718 -2if left untreated. Williams, et al., In: Harrison’s Principles of Internal Medicine, 10th ed., Petersdorf, R.G., et al., eds., McGraw-Hill, New York, 1983, chapter 247.
Hypertensive patients are classified into two broad categories. A relatively small proportion of patients develop hypertension as a manifestation of an identifiable and specifically treatable underlying cause, such as pheochromocytoma, renal arterial stenosis, or endocrine disease. Such patients are denominated as exhibiting secondary hypertension.
From 80-95% of patients with sustained elevated arterial pressure have no discernible underlying causative factor.
These patients are said to have essential or primary or idiopathic hypertension.
The etiology of essential hypertension is almost certainly multifactorial. Such factors include the sympathetic and parasympathetic nervous systems, baroreceptor function, and the renin-angiotensin-aldosterone and the atrial natriuretic factor systems that control renal sodium and water flux. In I addition, genetic and cardiovascular risk factors such as obesity, hypercholesterolemia, diabetes, salt intake, occupation, and cigarette smoking have long been assumed to be important in the genesis of essential hypertension.
Whatever the etiology of the disease, patients with untreated hypertension die prematurely, most commonly due to heart disease, but with strokes and renal failure often frequently occurring. As a result of clinical trials, there is firm evidence that reducing diastolic blood pressure that is above 90 mm Hg in middle-aged patients is associated with a decrease in cardiovascular morbidity and mortality. Blaschke, et al., In: Goodman and Gilman’s The Pharmacological Basis of Therapeutics, Gilman, et al., eds., McMillan, New York, 1980, chapter 32.
WO 89/07948 PCT/US89/00718 and collecting the precipitate which contains the Milk Anti- Hypertensive Factor (abbrev. MAF); and lyophilizing the ii I I «WO 89/07948 PCT/US89/00718 -3- Current treatments of hypertension consist of general measures and specific measures. General measures include dietary control and elimination of cardiovascular risk factors such as overweight, cigarette smoking, high salt intake, stress, etc.
Specific measures for treating hypertension consist of therapy with antihypertensive drugs. Many clinical studies have demonstrated that in almost all cases of hypertensive cardiovascular disease, the blood pressure can be successfully controlled by skillful use of the drugs currently available.
However, it has also been estimated that only a small percentage of all hypertensive patients under treatment are even close to optimal control. Thus, effective use of antihypertensive drugs is not «routine,» and therapy must be tailored to the individual patient and be adjusted as necessary to maintain an optimal balance between the therapeutic effects and the well-known adverse side effects associated with currently-employed antihypertensive drugs. Blaschke et al., supra at 809.
There are four classes of hypertensive drugs currently being used, each directed to a different mechanism of disease.
These classes include: diuretics, anti-adrenergic agents, vasodilators, and angiotensin blockers. Each is therapeutically effective in appropriate patients, but each may produce severe adverse side effects; all are expensive.
The popular thiazine diuretics are quickly effective, but may produce undesirable side effects such as hypokalemia, hypouricemia, and carbohydrate intolerance. Anti-adrenergic drugs «beta blockers») are effective in certain types of hypertension, when there is increased cardiac sympathetic nerve activity, but such drugs may precipitate congestive heart failure and asthma, and must be used with caution in diabetics; other side effects include dizziness, WO 89/07948 PCT/US89/00718 -4depression, nausea, vomiting, diarrhea, and constipation.
Vasodilators, which act primarily on arterial resistance rather than on venous capacitance, are effective against hypertension, but the effects of such agents are unfortunately negated by reflex increases in sympathetic discharges that raise heart rate and cardiac output. Thus, vasodilators are contraindicated in severe coronary artery disease, and are also known to produce undesirable side effects such as headache, tachycardia, angina pectoris, anoxia, nausea, vomiting, diarrhea, and a lupus-like syndrome. Angiotensin blockers are promising, but frequent side effects include leukopenia, pancytopenia, proteinuria, nephrotic syndrome, rash, fever, and loss of taste. Williams et al., supra at 1485.
In all but the mildly hypertensive patient who responds favorably to a diuretic or to a beta blocker, combinations of drugs are used. A successful combination is one whose components give additive or synergistic therapeutic effects, while causing minimal toxicity. Ideally, therapy should lower blood pressure effectively without producing side effects that become so unacceptable to the patient that compliance to the regimen is threatened or that compromise of the vital functions of the brain, heart, or kidneys.
A very difficult and important problem in the drug therapy of hypertension is to determine what constitutes «acceptable» side effects. All the effective antihypertensive drugs can produce quite significant adverse effects, to the extent where, in clinical trials, patients reported feeling better with the placebo than with the antihypertensive drug.
Blaschke et al., supra at 809.
In view of these shortcomings, the limitations to the antihypertensive drug therapy known to the prior art are readily apparent. It would be highly desirable, therefore, to ,iI i- -P WO 89/07948 PCT/US89/00718 have an antihypertensive agent that is inexpensive, readily available, derived from a natural source, easily administered, highly effective against hypertension, and devoid of undesirable side effects. The hyperimmune bovid milk of the present invention is such an antihypertehsive agent.
It has been known in the prior art to proauce milk having a variety of therapeutic effects. Beck, for example, has disclosed a milk containing antibody to Staphvlococcus mutans that has dental caries-inhibiting effects (Beck, U.S. Patent 4,324,782; British Patent 1,505,513). The milk is obtained by hyperimmunization of a cow with Streptococcus mutans antigen and collecting the therapeutic milk therefrom. Beck has also described a milk having anti-arthritic properties Patent 4,732,757), and has patented a method of treating inflammation using milk from hyperimmunized cows (Beck, U.S. Patent 4,284,623). Stolle has disclosed a method of using milk from a hyperimmunized cow to treat diseases of the vascular and pulmonary systems (Stolle et al., U.S. Patent 4,636,384).
Heinbach, U.S. Patent 3,128,230, has disclosed milk containing alpha, beta and gamma globulins against anti-9-enic haptens.
Singh Patent 3,911,108), Peterson Patent 3,376,198 and Canadian Patent 587.849), Holm Application (published) Serial No. 628,987), Tannah et al. (British Patent 1,211,876), and Biokema S.A. (British Patent 1,442,283) have also described antibody-containing milks. Steffkoff et al., Minerva Medica (Italy) 56:4163-4 (1965) have described the treatment of hypertensive diseases with sour milk.
It is well known to those skilled in the art of immunology that serum globulin fractions consisting of various antibody types such as IgA, IgG, and IgM can be used to counter the corresponding antigens, thereby neutralizing the effects of the antigens. There is an almost infinite number of harmful antigens to which animals can be exposed, including i’ WO 89/07948 PCT/US89/00718 -18example, in U.S. 3,773,919; U.S. 3,887,699; U.S. 4,118,470; U.S. 4,076,798; all incorporated by reference herein. The WO 89/07948 PCT/US89/00718 I -6carcinogenic, bacterial, viral, and regulatory factors of plant and animal origin, as well as toxins and poisons.
Normally, upon exposure to a foreign antigen, a bacterium, the immune system of the host will produce antibodies that will neutralize the effects of the antigen.
Exposure to such foreign antigens can occur either naturally, or deliberately by administration of the antigen in vaccine form. The latter is generally referred to as active immunization -of the host species exposed to the antigen. The antibodies produced in response to such vaccination are homologous to said given species of animal, and are epitopic to the antigen.
In copending related patent applications, U.S. Patent 4,636,384, and U.S. Application No. 001,848, filed January 9, 1987, there was disclosed a method of lowering blood lipid levels and treating lipid-associated vascular disorders, as well as treating macrophage-related pulmonary disorders, comprising feeding test animals and humans antibody-containing milk derived from cows maintained in a hyperimmune state by injections of polyvalent antigens derived from mixtures of killed bacteria. In another copending application (U.S.
Application No. 069,139, filed July 2, 1987), there was disclosed use of antibody-containing milk derived from hyperimmunized cows for the treatment of gastrointestinal disorders. The present invention is a further development over the inventions disclosed and claimed in the aforementioned applications, the entire disclosures of which are herein incorporated by reference.
All of these references, however, relate only to the isolation from hyperimmunized animals of immunoglobulins raised against various antigens and to the subsequent use of said antigens for either diagnostic procedures, homologous or heterologous passive immunization, or active immunization. No 7 suggestion or speculation is made in any of these references that milk derived from hyperimmunized bovids is useful in lowering arterial blood pressure.
SUMMARY OF THE INVENTION The present inventors have previously disclosed hyperimmune bovid milk with anti-inflammatory effects and with beneficial effects against vascular, pulmonary, and gastro-intestinal disorders.
The beneficial vascular effects of the hyperimmune bovid milk prompted the present inventors to investigate possible effects of the milk on elevated arterial plessure in humans and other animals.
With this in mind, the inventors then made the unexpected discovery that milk obtained from cows hyperimmunized against certain bacterial antigens the hyperimmune milk of the invention), when administered orally to hypertensive subjects, dramatically decreased the blood 15 pressure to normotensive values, and did so without any detectable undesirable side effects. It was also discovered that milk of non-hyperimmunized cows also contains anti-hypertension activity, but that this activity is greatly enhanced by the hyperimmunization of the donor cow.
20 Therefore, the invention relates to a method of production of hyperimmune bovid milk, a composition of said milk suitable for administration to a subject, and to the use of said composition to reduce elevated arterial pressure in such subjects.
According to a first embodiment of this invention, there is provided an anti-hypertensive factor, as herein defined, in substantially pure form, produced by a process comprising: obtaining milk from a hyperimmunized animal; (ii) removing from said milk macromolecules having a molecular weight greater than about 10,000 daltons; (iii) fractionating by ion-exchange chromatography the lower-than- L 10,000 dalton product of step (ii) to obtain a negatively charged product of molecular weight less than about 10,000 daltons; (iv) fractionating by molecular sieve chromatography said negatively charged product from step (iii); and isolating by isoelectric precipitation said anti-hypertensive product fractionated in step (iv).
S, :1295v \4!T 0>’ t 7a According to a second embodiment of this invention, there is provided a method of isolating a substantially pure milk anti-hypertensive factor, as herein defined, comprising: obtaining milk from a hyperimmunized animal; (ii) removing from said milk macromolecules of molecular weight greater than 10,000 daltons; (iii) fractionating by ion-exchange chromatography the lower-than- 10,000 dalton molecular weight product of step (ii) to obtain a negatively charged product of molecular weight less than about 10,000 daltons; (iv) fractionating by molecular sieve chromatography said negatively charged product from step (iii); and isolating by isoelectric precipitation said anti-hypertensive product fractionated in step (iv).
According to a third embodiment of this invention, there is provided a method of treating hypertension in an animal requiring such treatment, comprising administering to said animal an anti-hypertensively effective amount of the milk anti-hypertensive factor of the first embodiment.
S:1295v p 1 T s 4 I WO 89/07948 PT/US89/00718 -8- BRIEF DESCRIPTION OF THE DRAWINGS A more complete appreciation of the invention and many of the attendant advantages thereof will be readily obtained as the same becomes better understood by reference to the following detailed description when considered in connection with the accompanying drawings, wherein: FIGURE 1 shows the mean percent change in systolic blood pressure in human subjects drinking control milk and hyperimmune milk.
FIGURE 2 shows the effect on human systolic blood pressure with time following intrabuccal absorption of a 5 mg dose of substantially pure milk anti-hypertensive factor.
FIGURE 3 shows the profile of elution with a salt gradient of milk antihypertensive factor from a DEAE-Sepharose CL-6B ion-exchange column (Step 2).
FIGURE 4 shows the profiles of elutions with water of milk antihypertensive factor from a Sephadex G-10 molecular sieve column (Step 3).
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The invention comprises a natural food product (milk) that has anti-hypertensive properties, a method for producing the same, and a method of use in treating subjects with elevated arterial pressure. The invention represents a significant advance in the state of the art of anti-hypertensive pharmacology because the product of this invention has no adverse side effects. Anti-hypertensive milk can be used to treat hypertension associated with any disease in animals and humans without fear of side effects because it is .a natural food product. Examples of human disease conditions which may be treated with anti-hypertensive milk include: WO 89/07948 PCT/US89/00718 -22- ISOLATION AND PURIFICATION OF MILK ANTIHYPERTENSIVE FACTOR S WO 89/07948 PCT/US89/00718 -9primary or secondary hypertension of whatever etiology. By the term «hypertension» is intended, for the purpose of this invention, elevated diastolic arterial pressure of at least mm Hg. By the term «anti-hypertensive factor» is intended a factor obtained in hyperimmune milk with arterial pressure lowering activity. Hyperimmunization of the milk-producing animal provides for a supranormal concentration of antihypertensive factor in the milk sufficient to enable its use therapeutically. By the term «hyperimmune milk» is intended, for the purpose of this invention, milk obtained from milkproducing animals maintained in a hyperimmune state, the details for hyperimmunization being described in greater detail below. By the term «supranormal concentration of antihypertensive factor» is intended a concentration greater than existing in the milk of a non-hyperimmunized animal.
By the term «milk-producing animal» is intended, for the purpose of this invention, mammals that produce milk in commercially feasible quantities, preferably cows, sheep, and goats, more preferably dairy cows of the genus Bos (bovid), particularly those breeds giving the highest yields of milk, such as Holstein.
By the term «bacterial antigen» is intended, for the purpose of this invention, a lyophilized preparation of heatkilled bacterial cells.
By the term «treating» is intended, for the purpose of this invention, that the symptoms of the disorder be ameliorated or completely eliminated. By the term «administered» is intended, for the purpose of this invention, any method of treating a subject with a substance, such as orally, intranasally, parenterally (intravenously, intramuscularly, or subcutaneously), or rectally, in a pharmaceutically acceptable Svehicle. By the term «animal» is intended, for the purpose of WO 89/07948 PCT/US89/00718 the invention, any living creature that is subject to hypertension, including humans and animals.
The invention is based on the’discovery that when a bovid is brought to a specific state of immunization by means of periodic booster administrations of an antigen or mixture of antigens, the bovid will produce milk which has highly beneficial properties in the treatment of hypertension. The beneficial milk properties are enhanced when the bovids are immunized. The induction of immune sensitivity alone is insufficient to enhance the antihypertensive properties in milk, as is shown by the fact that normal cows’ milk contains a lower level of activity than milk from hyperimmunized cows, even though cows have become sensitized against various antigens during normal immunization against cow diseases.
The anti-hypertensive properties of milk are maximized in milk produced by bovids maintained in the immune state by booster injections. Hyperimmunization is achieved by administering periodic boosters with sufficiently high doses of antigens or mixtures of antigens. The preferred dose range should be equal to or greater than 50% of the dosage necessary to cause primary sensitization of the bovid. Thus, there is a booster dosage threshold below which the maximum properties are not produced in the cow, even though the cow may be in what is normally called an immune state. In order to achieve the hyperimmune state, it is essential to test the bovid’s milk after a first series of booster administrations. If the milk does not contain the desired properties, a second series of boosters of higher dosage has to be administered. This process is repeated until the maximum properties appear in the milk. The process of producing the hyperimmune milk has been Sdisclosed in U.S. Patent 4,284,623 to Beck, which is herein incorporated by reference to the extent that it provides te i I -1 0 0 I WO 89/07948 PCT/US89/007i8.
-lldetails of the production of hyperimmune milk. In summary, the process comprises the following steps: 1. Antigen selection.
2. Sensitization of the bovid by primary immunization.
3. Testing the serum of the bovid to confirm sensitivity induction.
4. Administering boosters of appropriate dosage to induce and maintain a hyperimmune state.
Testing anti-hypertensive properties of the milk.
6. Collecting milk from the bovid during the hyperimmune state. The milk of the invention can be collected by conventional methods; however, special processing is necessary to protect the biological properties of the milk. Low temperature pasteurization is required; the pasteurization temperature should not exceed 140’C. Following pasteurization, the fat is removed by standard procedures. The milk is concentrated and spray-dried. The skim milk is concentrated under vacuum at low temperatures so as not to destroy the anti-hypertensive factor. (See e.g. Kozakowski, «Cheese and Fermented Milk Products», 2d Edition, 1977). The final product is a milk powder which has anti-hypertensive properties.
Fluid milk can also be used, of course, as well as concentrated milk products or a fraction of the milk containing the biologically-active factor, such as the acid whey fraction. By the term «whey» is intended, for the purpose of this invention, milk from which cream and caseinaceous material has been separated.
The milk of the invention can be provided in any amount which affects the decrease of hypertensive conditions in animals. Daily amounts, equivalent to 1 ml to 10 liters of whole fluid milk, can be provided, depending on the particular circumstances of the hypertension and the animal species. The i:
I
S
i WO 89/07948 PCT/US89/00718 -12preferred dose is 0.5-1.0 g of skim milk powder per kg of body weight.
The fat-free milk can, of course, be incorporated into any food product as long as the food product is not treated at a temperature which is too elevated and would inactivate the anti-hypertensive properties of the product. A temperature lower than 140’C is preferred. For example, puddings or yogurt may be prepared with anti-hypertensive milk.
Further, when the fat-free milk is treated with rennet, and the casein is separated after precipitation thereof, it is found that the sweet-whey supernatant fraction contains the anti-hypertensive factor. This sweet-whey factor may also be added to syrups, ice cream mixes, candy, beverages, cattle feeds or the like. (See, Kozakowski, supra, at 446).
The process of producing the hyperimmune milk containing supranormal levels of anti-hypert asive factor is as follows.
In summary, one process of producing the hyperimmune milk containing supranormal levels of anti-hypertensive factor comprises the following steps: antigen selection; (2) primary immunization of the bovid; testing the serum to confirm sensitivity induction; hyperimmunization with boosters of appropriate dosage; and optionally, testing the milk for anti-hypertensive properties; collecting the milk from the hyperimmune bovid; and processing the milk to fractionate the anti-hypertensive factor.
Step 1: Any antigen o combination of antigens may be employed. The antigens can be bacterial, viral, protozoan, fungal, cellular or any other substances to which the immune system of a milk-producing animal will respond. The critical point in this step is that the antigen(s) must be capable, not only of inducing immune and hyperimmune states of the milk producing animal, but also of producing supranormal levels of anti-hypertensive factor in the milk. Preferably, polyvalent ,-a.a
I-
WO 89/07948 PCT/US89/007i8 -13bacterial agents are used. One preferred vaccine is a mixture of polyvalent bacterial antigens, described in detail in Example I below.
Step 2: The antigen(s) can be administered in any method that causes sensitization. In one method, a vaccine composed of antigen derived from 1 x 106 to 1 x 1020, preferably 108 to 1010, most preferably 2 x’ 108, heat-killed bacteria is administered by intramuscular injection. However, other methods such as intravenous injection, intraperitoneal injection, rectal suppositories, or oral administration may be used.
Step 3: It is necessary to determine whether or not the milk-producing animal has become sensitive to the antigen.
There are a number of methods known to those skilled in the art of immunology to test for sensitivity (Methods in Immunol- OQy and Immunochemistry, Williams, C.A. et al., Academic Press, New York, Volumes 1-5 (1975)). The preferred method is to use a polyvalent vaccine comprising multiple bacterial species as the antigen and to test for the presence of agglutinating antibodies in the serum of the animal before and after challenge with the vaccine. The appearance of milk antibodies after immunization with the vaccine indicates sensitivity; at this point, it is possible to proceed to step 4.
Step 4: This involves the induction and maintenance of the hyperimmune state in the sensitized animal. This is accomplished by repeated booster administration at fixed time intervals of the same polyvalent vaccine that was used to achieve the primary sensitization. A two-week booster interval is optimal for polyvalent bacterial antigens.
However, it is necessary to insure that the animal does not pass from a hyperimmune state to a state of immune tolerance to the antigen.
WO 89/07948 PCT/US89/00718 -14- In a preferred embodiment, hyperimmunization of bovids may be achieved by a single administration of microencapsulated vaccine, prepared as described in detail in Example 1B below.
In an alternative embodiment, it is possible to combine different immunization procedures, simultaneously administering microencapsulated and liquid antigen, or intramuscular injection for primary immunization, and booster doses by oral administration or parenteral administration by microencapsulation means. Many different combinations of primary and hyperimmunization are known to those skilled in the art.
Step 5: It is necessary to test the milk for antihypertensive activity levels. This can be accomplished by any research technique that tests the effects of either the hyperimmune milk or products derived therefrom upon hypertension.
Step 6: This involves the collection and processing of the milk. The milk can be collected by conventional methods.
Fractionating the milk to obtain the anti-hypertensive factor can t»‘n be carried out.
Step 7: The general method of isolation and purification of the anti-hypertensive factor from hyperimmune milk is as follows: the milk is defatted to produce skim milk; (b) optionally, casein is removed from the skim milk to produce whey; macromolecules of molecular weight 10,000 daltons are removed from the whey; the product from the previous step is fractionated on an ion-exchange column to obtain negatively-charged molecules having molecular weights 10,000 daltons; the milk anti-hypertensive factor-containing, <10,000 dalton fraction can be size fractionated on a molecular sieve gel; increasing the pH of the <10,000 dalton fraction to alkaline values of about 9 to 12, preferably 10.5, WO 8/07948 PCT/US89/00718 and collecting the precipitate which contains the Milk Anti- Hypertensive Factor (abbrev. MAF); and lyophilizing the precipitate from the previous step. Tha invention is based in part upon the unexpected discovery that an anti-hypertensive factor, found normally in milk but produced in large quantities by hyperimmunizing a milk-producing animal against a bacterial antigen vaccine, is effective in treating a variety of hypertensive processes in humans and animals, including dogs. The vaccine used to hyperimmunize the animals does not contain anti-hypertensive activity. It is surprising therefore, that treatment with a milk factor, obtained from animals immunized against a mixed killed bacterial antigen vaccine, is effective in elevating or eliminating hypertensive processes. Having now described the invention in general terms, the same will be further described by reference to certain specific examples that are provided herein for purposes of explanation only, and are not intended to be limiting unless otherwise specified. PREPARATION OF MILKS EXAMPLE IA Preparation of Mixed Bacterial Vaccine A bacterial culture, containing the spectrum of bacteria shown in Table 1 below as obtained from the American Type Culture Collection, was reconstituted with 15 ml of growth medium and incubated overnight at 37*C. Once good growth was obtained, approximately one-half of the bacterial suspension was employed to inoculate one liter of broth, with the inoculate being incubated at 37'C. The remaining suspension :w SWO 89/07948 PCT/US89/00718 -16was transferred to sterile glycol tubes and stored at for up to six months. After good growth was visible in the culture, bacterial cells were harvested by centrifugation of the suspension for minutes to remove the growth medium. The bacterial pellet obtained was resuspended in sterila saline solution and the bacterial sample was centrifuged three times to wash the medium from the cells. After the third sterile saline wash, the bacterial pellet obtained upon centrifugation was resuspended in a small amount of double distilled water. The medium-free bacterial suspension was heat-killed by placing the suspension in a glass flask in an 80°C water bath overnight. The viability of the broth culture was tested with a small amount of heat-killed bacteria, as follows: broth was inoculated with heat-killed bacteria, incubated at 37°C for five days and checked daily for growth. For the safety of the cow, the bacteria should be killed for use in the vaccine. The heat-killed bacteria were lyophilized until dry. The dry bacteria were then mixed with sterile saline solution to a concentration of about 2.2 x 108 bacterial cells/ml saline optical density units at 660 nm). Table 1 Bacteria List Gram Name Media or ATTC I. Staph. aureus BHI 11631 2. Staph. epidermidis BHI 155 3. Strep. pyogenes, A. Type 1 APT 8671 4. Strep. pyogenes, A. Type 3 APT 10389 Strep. pyogenes, A. Type 5 APT 12347 6. Strep. pyogenes, A. Type 8 APT 12349 7. Strep. pyogenes, A. Type 12 APT 11434 8. Strep. pyogenes, A. Type 14 APT 12972 9. Strep. pyogenes, A. Type 18 APT 12357 Strep. pyogenes, A. Type 22 APT 10403 3 ;r~ -4: %WO 89/07948 PCT/US89/00718 -17- 11. Aerobacter aerogenes BHI 884 12. Escherichia coli BHI 26 13. Salmonella enteritidis BHI 13076 14. Pseudomonas aeruginosa BHI 7700 Klebsiella pneumoniae BHI 9590 16. Salmonella typhimurium BHI -13311 17. Haemophilus typhimurium BHI 9333 18. Strep. mitis APT *6249 19. Proteus vulgaris BHI 13315 Shigella dysenteriae BHI 11835 21. Diplococcus pneumoniae APT 6303 22. Propionibacter acnes Actinomyces (anaerobe) Broth 11827 23. Strep. sanguis APT 10556 24. Strep. salivarius APT 13419 Strep. mutans BHI 25175 26. Strep. agalactiae APT 13813 Cows were given daily injections of 5 ml samples of the polyvalent liquid vaccine. Antibody (IgG) titer levels for the injected cattle were determined periodically by taking optical density readings at 410 nm of antibody-containing fluid samples obtained from cow's milk. Example 1B Preparation of Controlled Release Mixed Bacterial Vaccine. Heat-killed bacteria were prepared in the manner described above. The polyvalent antigen obtained was microencapsulated by a conventional phase-separation process to prepare a polyvalent antigen-containing microparticle product. Generally, the antigen-containing shaped matrix materials are formed from polymers of biocompatible material, preferably biodegradable or bioerodable materials, preferably polylactic acid, polyglycolic acid, copolymers of lactic and glycolic acids, polycaptolactone, copolyoxalates, proteins such as collagen, I fatty acid esters of glycerol, and cellulose esters. These polymers are well known in the art and are described, for __i i WO 89/07948 PCT/US89/00718 -18example, in U.S. 3,773,919; U.S. 3,887,699; U.S. 4,118,470; U.S. 4,076,798; all incorporated by reference herein. The polymeric matrix material employed was a biodegradable lactide-glycolide copolymer. Heat-killed bacterial antigens are encapsulated in such matrix materials., preferably as microsphereA of between 1-500 microns diameter, preferably 10-250 microns. The encapsulation processes are conventional and comprise phase separation methods, interfacial reactions, and physical methods. Many combinations of matrices and many concent'ations of assorted antigens may be employed, in order to provide for optimal rates of release of bacterial antigens to the host body from the microparticles. These combinations can be determined by those skilled in the art without undue experimentation. The microparticles in the example were less than 250 microns in diameter. Approximately 750 mg of microparticles containing 22% (16.5 mg) of polyvalent antigen was then suspended in about 3 cc of a vehicle (1 wt Tween 20 and 2 wt carboxymethyl cellulose in water). A small group of cattle was selected from a larger herd of cattle. Five of these randomly selected cattle were selected as controls. Four cattle were treated by an injection of microparticle-containing solution prepared as described above. The four cows were injected intramuscularly with the polyvalent antigen-containing microparticles suspended in saline. The dose of antigen was over 2 x 108 bacterial cells per ml. Antibody (IgG) titer levels were determined periodically from samples of cows' milk obtained from the inoculated cows, as well as from the control cows. "P WO 89/07948 Preparation of PCT/US89/00718 -19- Example 1C Vaccine of Unknown Antigen Composition Bt We have discovered that bee honey contains a diverse group of antigens, the composition of which is unknown. The antigen in honey when injected in cows produces a hyperimmune state that results in maximum hypertensive properties of milk. For example, a dose of 1 ml of honey diluted 1:5 in water was injected into 5 cows, once a week for 5 consecutive weeks, then once every 2 weeks for 6 months. Milk was collected between the third and sixth months for testing. This example is included to illustrate the points that the exact composition of the antigen need not be known to practice the invention, and that any antigen or combination of antigens either known or unknown might be used to immune the cows for the production of the antihypertensive factor. I i TESTING OF HYPERIMMUNE MILK FOR ANTIHYPERTENSIVE ACTIVITY Example 2 This was a double blind controlled study in which one experimental group of seven human volunteer subjects was fed hyperimmune milk product and a second control group of seven subjects was fed milk produced from nonhyperimmunized cows. Systolic blood pressures were measured both before and during treatment. The study was divided into two phases, with a total duration of 14 weeks. The first phase was a 4-week pretreatment phase, at the end of weeks 1 and 4 of which systolic blood pressures were determined. The second phase consisted of a 10-week treatment period during which subjects consumed ~clc--- WO 89/07948 PCT/US89/00718 daily 50 g of powdered skim milk suspended in water, with blood pressure measurements being made at the end of weeks 2, 4, 6, 8 and 10. Those skilled in the art will know of pharmaceutically acceptable vehicles in which to suspend forms 'of milk protein. The results of this study are shown both in Table 1 and Figure 1, with Table 1 providing the raw data and Figure 1 providing a comparison of the mean percent change in systolic blood pressure between the experimental and control groups. Systolic blood pressures of subjects ingesting the hyperimmune milk product decreased with time over the 10-week treatment period, with the decrease ranging between 5.3% to 8.40%. In contrast, with the control group fed non-hyperimmune milk product the changes were much smaller, ranging between 1% and 3.92% over the 10-week treatment period, but these changes were also related to length of treatment. Table 1 IMMUNE MILK GROUP WEEK PATIENT -3 0 2 4 6 8 1 135 128 128 124 118 110 116 2 128 126 120 118 118 116 108 4 120 125 118 115 1.10 106 112 18 150 124 124 127 127 130 120 19 128 138 126 130 128 128 122 21 116 110 115 100 100 112 116 7 118 105 98 98 108 102 108 MEAN 127.86 122.29 116.00 117.00 114.86 114.57 change 0.00 0.00 5.31 7.25 6.45 8.17 8.40 ir i' H I WO 89/094 PCT/US89/00718 -21- NORMAL MILK GROUP 3 135 130 125 120 128 118 114 118 118 117 120 116 115 118 11 105 118 116 102 114 100 108 13 108 110 100 102 110 113 99 16 116 110 118 114 108 105 118 17 130 130 130 138 132 136 130 115 116 115 112 108 106 110 MEAN 118.14 118.86 117.29 115.43 116.57 113.29 113.86 change 0.00 0.00 1.02 2.59 1.63 4.40 3.92 Example 3 In this experiment, 5 mg of partially purified MAF test material (see Example 4 for details of the isolation and purification of MAF) were placed directly on the tip of the tongue of a human volunteer subject, and systolic blood pressure determinations were made at 10-to 15-minute intervals over the next hour. The results of one such experiment are shown in Figure 2. The systolic blood pressure-lowering effect was immediate and significant, causing a 30-40% drop in pressure within 30 minutes. This experiment was repeated several times with milk MAF obtained from cows treated with different antigens (see Figures 1A-IC). The results in each case were similar. This procedure may be used for rapid screening of various milk fractions for MAF activity. WO 89/07948 PCT/US89/00718 -22- ISOLATION AND PURIFICATION OF MILK ANTIHYPERTENSIVE FACTOR ExamDle 4 STEP 1: Milk Filtrate Preparation Twenty liters of fresh milk from normal or hyperimmunized cows were run through a cream separator (Delaval Model 102) to remove the fat. The resultin 16 liters of skimmed milk were ultrafiltered to remove the high molecular weight species (>10,000 daltons) using a hollow fiber diafiltration/concentrator (Amicon DL-IOL). The concentrator was equipped with two 10,000 dalton molecular weight cut-off cartridges. The skimmed milk was run at the pump speed of 80 on the meter and inlet and outlet pressure of 30 psi and 25 psi respectively.
Twelve liters of the filtrate (<10,000 daltons) coming out of the cartridges at the flow rate of four liters per hour was frozen or lyophilized for storage and for further purification. STEP 2: Ion-Exchange Chromatography The milk anti-hypertensive factor, MAF, in the filtrate was first isolated by an anion exchange chromatography column. In this procedure, DEAE-Sepharose CL-6B gel (Pharma.cia) was used to pack a 5xlOcm glass column which was equilibrated with sterile double distilled water, pH One liter of filtrate (<10,000 dalton fraction) was applied to the column and eluted with sterile double distilled water, pH 7.0, at the flow rate of 160 ml per hour. Ten milliliter fractions were collected and monitored at 280 nm in an LKB Uvicord 4700 absorptiometer with an optical density printed out on a connected recorder (Pharmacia REC-482). I"i i i IiWO 89/7948 PCT/US89/00718 -23- The substances other than MAF having positive and neutral charges are not bound to the DEAE-Sepharose gel. They were eluted at the fall-through peak (first peak). The MAF carrying a negative charge was retained by the gel. To elute the MAF, the column was eluted with a stepwise gradient using a sterile NaCl solution, pH 7.0. A typical profile is shown in Figure 3. The second peak and its shoulder contained the MAF and other negatively charged species. The 20 ml fractions containing the second peak and its shoulder were pooled and lyophilized for storage and for further purification. Recovery studies showed that seven grams of dried powder were obtained. STEP 3: Gel Filtration Chromatography The fraction obtained from Step 2 was composed of MAF and other negatively charged components; therefore, an additional refining step was needed. To achieve further purification, it was necessary to use a gel filtration column to separate those negatively-charged components on the basis of molecular size and shape. In this process, Sephadex G-10 resin (Pharmacia) was packed into a 2.5X80 cm glass column and equilibrated with sterile double distilled water, pH 7.0. Two grams of the MAF fraction from Step 2 were redissolved in sterile double distilled water and applied to the top of the column. The column was eluted at a flow rate of 30 ml per hour. Fractions of 3.3 ml were collected and monitored at 254 nm and 280 nm (Pharmacia Duo Optical Unit) with optical density printed out on a connected recorder (Pharmacia REC-482). There were 3 peaks in the elution profile, as shown in Figure 4. The last peak, which is freed of large negatively charged substances, contained the MAF. Recovery studies WO 89/07948 PCT/US89/00718 -24showed a yield of 260 mg of dry powder from the overall process. Having now generally described the invention, it will become readily apparent to those skilled in the art that many changes and modifications can be made thereto without affecting the spirit or scope thereof. Claims (19) 1. An anti-hypertensive factor, as herein defined, in substantially pure form, produced by a process comprising: obtaining milk from a hyperimmunized animal; (ii) removing from said milk macromolecules having a molecular weight greater than about 10,000 daltons; (iii) fractionating by ion-exchange chromatography the lower-than- 10,000 dalton product of step (ii) to obtain a negatively charged product of molecular weight less than about 10,000 daltons; (iv) fractionating by molecular sieve chromatography said negatively charged product from step (iii); and isolating by isoelectric precipitation said anti-hypertensive product fractionated in step (iv). 2. The milk anti-hypertensive factor of claim 1 wherein 15 said isoelectric precipitation is carried out at pH values above about 9 and below about 12. s 3. The milk anti-hypertensive factor of claim 1 wherein said isoelectric precipitation is carried out at a pH of about 10.5. 4. A method of isolating a substantially pure milk 20 anti-hypertensive factor, as herein defined, comprising: obtaining milk from a hyperimmunized animal; (ii) removing from said milk macromolecules of molecular weight greater than 10,000 daltons; (iii) fractionating by ion-exchange chromatography the lower-than- Si 25 10,000 dalton molecular weight product of step 1:1295v I: I I WO 89/07948 PCT/US89/00718 -26- (ii) to obtain a negatively charged product of molecular weight less than about 10,000 daltons; (iv) fractionating by molecular sieve chroma- tography said negatively charged product from step (iii); and isolating by isoelectric precipitation said antihypertensive product fractionated in step (iv). The method of claim 4 wherein said isoelectric precipitation is carried out at pH values above about 9 and below about 12. 6. The method of claim 4 wherein said isoelectric precipitation is carried out at a pH of about 10.5. 7. A method of treating hypertension in an animal reqL A\\r\g sua c r ea re V kcomprising administering to said animal an anti-hypertensively effective amount of the milk anti-hypertensive factor of any one of claims 1 o- +o 3. 8. The method of claim 7, wherein said hypertensive factor comprises whole milk. 9. The method of claim 7, wherein said hypertensive factor comprises whole milk powder. The method of claim 7, wherein said hypertensive factor comprises skim milk. 11. The method of claim 7, wherein said hypertensive factor comprises skim milk powder. 12. The method of claim 7, wherein said hypertensive factor comprises milk whey. milk anti- milk anti- milk anti- milk anti- milk anti- i I I WO 89/07948 P,.T/US89/00718 -27- 13. The method of claim 7, wherein said milk anti- hypertensive factor comprises a subfraction of milk whey. 14. The method of claim 7, wherein said milk anti- hypertensive factor comprises said substantially pure molecule of molecular weight below about 10,000 daltons. The method of claim 7, wherein said animal is a human. 16. The method of claim 7, wherein said milk anti- hypertensive factor is administered periodically to said animal. 17. The method of claim 7, wherein said administration of said ,nti-hypertensive milk factor is selected from among oral, rarenteral or intranasal means. 18. The method of claim 17, wherein said oral means comprises a suspension of .i44k form of said factor in a pharmaceutically-acceptable vehicle. 19. The method of claim 17, wherein said oral means comprises an encapsulated form of said factor, said encapsu- lated form being selected from among timed release and enteric coated capsular forms. The method of claim 17, wherein said parenteral means is selected from among intravenous, intraperitoneal, intramuscular and subcutaneous means. 7 28 21. factor is vehicle. 22. factor is vehicle. The method of claim 17, wherein said milk anti-hypertensive administered parenterally in a pharmaceutically acceptable The method of claim 17, wherein said milk anti-hypertensive administered intranasally in a pharmaceutically acceptable 23. An anti-hypertensive factor, as herein defined, substantially as herein described with reference to Example 3 or 4. 24. A method of isolating a substantially pure milk anti- hypertensive factor, as herein defined, which method is substantially as herein described with reference to Example 4. A method of treating hypertension in an animal requiring such treatment, comprising administering to said animal an anti-hypertensively effective amount of the milk anti-hypertensive factor as defined in claim 23. DATED this NINTH day of JANUARY 1992 Stolle Research and Development Corporation Patent Attorneys for the Applicant SPRUSON FERGUSON V d S -o W: 1295v r- I I AU33500/89A 1988-02-26 1989-02-23 Antihypertensive hyperimmune milk, production, composition, and use Ceased AU621604B2 (en) Applications Claiming Priority (2) Application Number Priority Date Filing Date Title US07/161,039 US4879110A (en) 1983-10-27 1988-02-26 Antihypertensive hyperimmune milk, production, composition, and use US161039 1988-02-26 Publications (2) Publication Number Publication Date AU3350089A AU3350089A (en) 1989-09-22 AU621604B2 true AU621604B2 (en) 1992-03-19 Family ID=22579548 Family Applications (1) Application Number Title Priority Date Filing Date AU33500/89A Ceased AU621604B2 (en) 1988-02-26 1989-02-23 Antihypertensive hyperimmune milk, production, composition, and use Country Status (15) Country Link US (1) US4879110A (en) EP (1) EP0330521B1 (en) JP (1) JP2792973B2 (en) AT (1) ATE88349T1 (en) AU (1) AU621604B2 (en) CA (1) CA1339760C (en) DE (1) DE68906054T2 (en) DK (1) DK201590A (en) ES (1) ES2039847T3 (en) HK (1) HK1005013A1 (en) IE (1) IE63032B1 (en) IL (1) IL89352A (en) MX (1) MX168997B (en) NZ (1) NZ228105A (en) WO (1) WO1989007948A1 (en) Cited By (1) * Cited by examiner, † Cited by third party Publication number Priority date Publication date Assignee Title AU648800B2 (en) * 1990-07-05 1994-05-05 Stolle Milk Biologics, Inc. 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Process for the purification of proteins from milk and from its by-products AU613688B2 (en) * 1987-11-27 1991-08-08 Campina Melkunie B.V. Process for extracting pure fractions of lactoperoxidase and lactoferrin from milk serum AU620289B2 (en) * 1988-04-04 1992-02-13 Stolle Milk Biologics, Inc. An anti-inflammatory factor, method of isolation, and use Cited By (1) * Cited by examiner, † Cited by third party Publication number Priority date Publication date Assignee Title AU648800B2 (en) * 1990-07-05 1994-05-05 Stolle Milk Biologics, Inc. Immune suppressive product Also Published As Publication number Publication date IL89352A0 (en) 1989-09-10 US4879110A (en) 1989-11-07 ES2039847T3 (en) 1993-10-01 IE890607L (en) 1989-08-26 EP0330521A2 (en) 1989-08-30 MX168997B (en) 1993-06-16 DE68906054T2 (en) 1993-09-16 NZ228105A (en) 1990-10-26 CA1339760C (en) 1998-03-17 EP0330521A3 (en) 1990-10-31 AU3350089A (en) 1989-09-22 IL89352A (en) 1994-06-24 EP0330521B1 (en) 1993-04-21 ATE88349T1 (en) 1993-05-15 HK1005013A1 (en) 1998-12-18 JP2792973B2 (en) 1998-09-03 DK201590D0 (en) 1990-08-22 WO1989007948A1 (en) 1989-09-08 IE63032B1 (en) 1995-03-22 DE68906054D1 (en) 1993-05-27 JPH03505866A (en) 1991-12-19 DK201590A (en) 1990-08-22 Similar Documents Publication Publication Date Title AU621604B2 (en) 1992-03-19 Antihypertensive hyperimmune milk, production, composition, and use EP0336694B1 (en) 1995-07-05 An anti-inflammatory factor from milk CA2090011C (en) 2003-12-30 Anti-inflammatory factor, method of isolation, and use CA2146442C (en) 2005-01-25 Anti-inflammatory factor, method of isolation, and use US5194255A (en) 1993-03-16 Antihypertensive hyperimmune milk, production, composition, and use US5980953A (en) 1999-11-09 Anti-inflammatory factor, method of isolation, and use US5650175A (en) 1997-07-22 Anti-inflammatory factor, method of isolation, and use WO1996010410A9 (en) 1996-05-23 Anti-inflammatory factor, method of isolation, and use US10716850B2 (en) 2020-07-21 Anti-inflammatory factor retentate, method of isolation, and use NZ505177A (en) 2005-01-28 Use of milk anti-Inflammatory factor (MAIF) to reduce tissue damage from inflammation
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