AU7119087A – Process for renaturing of proteins
– Google Patents
AU7119087A – Process for renaturing of proteins
– Google Patents
Process for renaturing of proteins
Info
Publication number
AU7119087A
AU7119087A
AU71190/87A
AU7119087A
AU7119087A
AU 7119087 A
AU7119087 A
AU 7119087A
AU 71190/87 A
AU71190/87 A
AU 71190/87A
AU 7119087 A
AU7119087 A
AU 7119087A
AU 7119087 A
AU7119087 A
AU 7119087A
Authority
AU
Australia
Prior art keywords
proteins
renaturing
renatured
solution
renaturation
Prior art date
1986-04-08
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU71190/87A
Other versions
AU583723B2
(en
Inventor
Stephan Fischer
Rainer Rudolph
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
1986-04-08
Filing date
1987-04-08
Publication date
1987-10-15
1987-04-08
Application filed by Boehringer Mannheim GmbH
filed
Critical
Boehringer Mannheim GmbH
1987-10-15
Publication of AU7119087A
publication
Critical
patent/AU7119087A/en
1989-05-04
Application granted
granted
Critical
1989-05-04
Publication of AU583723B2
publication
Critical
patent/AU583723B2/en
2007-04-08
Anticipated expiration
legal-status
Critical
Status
Ceased
legal-status
Critical
Current
Links
Espacenet
Global Dossier
Discuss
Classifications
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
C12N9/14—Hydrolases (3)
C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
C12N9/2462—Lysozyme (3.2.1.17)
C—CHEMISTRY; METALLURGY
C07—ORGANIC CHEMISTRY
C07K—PEPTIDES
C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
C12N9/0004—Oxidoreductases (1.)
C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
C12N9/14—Hydrolases (3)
C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
C12N9/14—Hydrolases (3)
C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
C12N9/6424—Serine endopeptidases (3.4.21)
C12N9/6456—Plasminogen activators
C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12Y—ENZYMES
C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
C12Y304/21—Serine endopeptidases (3.4.21)
C12Y304/21069—Protein C activated (3.4.21.69)
Abstract
The present invention provides a process for the renaturation of denatured proteins in solution in a renaturation buffer, wherein a solution is prepared of the protein to be renatured in the critical concentration in a selected buffer and, after formation of the folding intermediate, further protein to be renatured is added in the amount necessary for the achievement of the critical concentration.
AU71190/87A
1986-04-08
1987-04-08
Process for renaturing of proteins
Ceased
AU583723B2
(en)
Applications Claiming Priority (2)
Application Number
Priority Date
Filing Date
Title
DE19863611817
DE3611817A1
(en)
1986-04-08
1986-04-08
METHOD FOR RENATURING PROTEINS
DE3611817
1986-04-08
Publications (2)
Publication Number
Publication Date
AU7119087A
true
AU7119087A
(en)
1987-10-15
AU583723B2
AU583723B2
(en)
1989-05-04
Family
ID=6298240
Family Applications (1)
Application Number
Title
Priority Date
Filing Date
AU71190/87A
Ceased
AU583723B2
(en)
1986-04-08
1987-04-08
Process for renaturing of proteins
Country Status (20)
Country
Link
US
(1)
US4933434A
(en)
EP
(1)
EP0241022B1
(en)
JP
(1)
JPH0742307B2
(en)
KR
(1)
KR900005279B1
(en)
AT
(1)
ATE84541T1
(en)
AU
(1)
AU583723B2
(en)
CA
(1)
CA1304191C
(en)
CZ
(1)
CZ283304B6
(en)
DD
(1)
DD260280A5
(en)
DE
(2)
DE3611817A1
(en)
DK
(1)
DK173650B1
(en)
ES
(1)
ES2038969T3
(en)
FI
(1)
FI93842C
(en)
GR
(1)
GR3007605T3
(en)
HK
(1)
HK46896A
(en)
HU
(1)
HU198514B
(en)
IE
(1)
IE60064B1
(en)
IL
(1)
IL82091A
(en)
SK
(1)
SK249587A3
(en)
ZA
(1)
ZA872474B
(en)
Cited By (1)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
WO1992004382A1
(en)
*
1990-09-05
1992-03-19
Bunge (Australia) Pty. Ltd.
Solubilization of proteins in active forms
Families Citing this family (13)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
WO1989001484A1
(en)
*
1987-08-14
1989-02-23
Gebro Broschek Kg Pharmazeutische Fabrik
Process for intramolecular oxidation of two -sh groups in a peptide compound to a disulphide bridge, and peptide so obtained
DE3832898A1
(en)
*
1988-09-28
1990-04-12
Boehringer Mannheim Gmbh
PRAEPARATE OF EXPRESSED PLASMINOGEN ACTIVATOR IN PROKARYONS
DE3835350A1
(en)
*
1988-10-17
1990-04-19
Boehringer Mannheim Gmbh
ACTIVATION OF GENETICALLY MANUFACTURED ANTIBODY EXPRESSED IN PROKARYONS
DE3903581A1
(en)
*
1989-02-07
1990-08-16
Boehringer Mannheim Gmbh
FABRIC PLASMINOGEN ACTIVATOR DERIVATIVE
EP0545999B1
(en)
*
1990-08-20
1997-06-04
Novo Nordisk A/S
Process for the preparation of biologically active IGF-1 using IGF-1 having an amino-terminal extension
US5023323A
(en)
*
1990-09-12
1991-06-11
Monsanto Company
Method of somatotropin naturation
DE4037196A1
(en)
*
1990-11-22
1992-05-27
Boehringer Mannheim Gmbh
METHOD FOR REACTIVATING DENATURED PROTEIN
DE4139000A1
(en)
1991-11-27
1993-06-03
Boehringer Mannheim Gmbh
METHOD OF GENERATING BIOLOGICALLY ACTIVE SS-NGF
EP1077263A1
(en)
1999-07-29
2001-02-21
F.Hoffmann-La Roche Ag
Process for producing natural folded and secreted proteins by co-secretion of chaperones
US20050079526A1
(en)
*
2002-02-20
2005-04-14
Affinium Pharmaceuticals, Inc.
Methods and apparatuses for characterizing refolding and aggregation of biological molecules
PL2547699T3
(en)
2010-03-17
2017-04-28
Ratiopharm Gmbh
Method for obtaining biologically active recombinant human g-csf
WO2012104099A1
(en)
2011-02-04
2012-08-09
Glucometrix Ag
Process for the production of recombinant trypsin
CN106699836A
(en)
*
2017-03-07
2017-05-24
安徽鑫华坤生物工程有限公司
Dialysis equipment for protein renaturation
Family Cites Families (13)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
US4216141A
(en)
1978-07-19
1980-08-05
The Salk Institute For Biological Studies
Method for cyclization of peptides
DE3231658A1
(en)
1981-01-21
1983-11-17
NZ204477A
(en)
*
1982-06-07
1986-09-10
Celltech Ltd
Recombinant dna method for production of chymosin:initial solubilisation of insoluble precursor
JPS593711U
(en)
*
1982-06-30
1984-01-11
協伸工業株式会社
Wire sheath stripper
AU538170B2
(en)
*
1982-09-15
1984-08-02
Owens-Illinois Glass Container Inc.
Modified protein to mould an enzyme type molecule
GR79124B
(en)
*
1982-12-22
1984-10-02
Genentech Inc
EP0122080B1
(en)
*
1983-03-25
1989-09-13
Celltech Limited
A process for the production of a protein
US4530787A
(en)
*
1984-03-28
1985-07-23
Cetus Corporation
Controlled oxidation of microbially produced cysteine-containing proteins
KR860007688A
(en)
1985-03-08
1986-10-15
시끼 모리야
Electromagnet Device
GB8508340D0
(en)
*
1985-03-29
1985-05-09
Creighton T E
Production of protein
EP0208539A3
(en)
*
1985-07-11
1988-08-03
Repligen Corporation
Folding disulfide-cross-linkable proteins
US4656255A
(en)
*
1985-09-09
1987-04-07
International Minerals & Chemical Corp.
Protein recovery
DE3618817A1
(en)
*
1986-06-04
1987-12-10
Behringwerke Ag
METHOD FOR OBTAINING ACTIVE PROTEINS FROM A BIOLOGICALLY INACTIVE FORM
1986
1986-04-08
DE
DE19863611817
patent/DE3611817A1/en
not_active
Withdrawn
1987
1987-03-16
IE
IE69587A
patent/IE60064B1/en
not_active
IP Right Cessation
1987-04-02
IL
IL82091A
patent/IL82091A/en
not_active
IP Right Cessation
1987-04-02
CA
CA000533687A
patent/CA1304191C/en
not_active
Expired – Lifetime
1987-04-06
DD
DD87301550A
patent/DD260280A5/en
not_active
IP Right Cessation
1987-04-06
JP
JP62083132A
patent/JPH0742307B2/en
not_active
Expired – Lifetime
1987-04-07
KR
KR1019870003281A
patent/KR900005279B1/en
not_active
IP Right Cessation
1987-04-07
HU
HU871493A
patent/HU198514B/en
unknown
1987-04-07
SK
SK2495-87A
patent/SK249587A3/en
unknown
1987-04-07
ZA
ZA872474A
patent/ZA872474B/en
unknown
1987-04-07
CZ
CS872495A
patent/CZ283304B6/en
not_active
IP Right Cessation
1987-04-07
FI
FI871522A
patent/FI93842C/en
not_active
IP Right Cessation
1987-04-08
DK
DK198701797A
patent/DK173650B1/en
active
IP Right Grant
1987-04-08
EP
EP87105230A
patent/EP0241022B1/en
not_active
Expired – Lifetime
1987-04-08
AU
AU71190/87A
patent/AU583723B2/en
not_active
Ceased
1987-04-08
AT
AT87105230T
patent/ATE84541T1/en
not_active
IP Right Cessation
1987-04-08
ES
ES198787105230T
patent/ES2038969T3/en
not_active
Expired – Lifetime
1987-04-08
DE
DE8787105230T
patent/DE3783497D1/en
not_active
Expired – Lifetime
1989
1989-01-13
US
US07/298,274
patent/US4933434A/en
not_active
Expired – Lifetime
1993
1993-04-09
GR
GR920403237T
patent/GR3007605T3/el
unknown
1996
1996-03-14
HK
HK46896A
patent/HK46896A/en
not_active
IP Right Cessation
Cited By (1)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
WO1992004382A1
(en)
*
1990-09-05
1992-03-19
Bunge (Australia) Pty. Ltd.
Solubilization of proteins in active forms
Also Published As
Publication number
Publication date
CA1304191C
(en)
1992-06-23
ES2038969T3
(en)
1993-08-16
IE870695L
(en)
1987-10-08
ATE84541T1
(en)
1993-01-15
DE3783497D1
(en)
1993-02-25
KR870010185A
(en)
1987-11-30
FI93842B
(en)
1995-02-28
FI871522A0
(en)
1987-04-07
HU198514B
(en)
1989-10-30
DE3611817A1
(en)
1987-10-15
DK173650B1
(en)
2001-05-21
DK179787D0
(en)
1987-04-08
IL82091A
(en)
1991-12-15
ZA872474B
(en)
1988-07-27
EP0241022A3
(en)
1989-10-25
IL82091A0
(en)
1987-10-30
CZ283304B6
(en)
1998-02-18
SK279976B6
(en)
1999-06-11
IE60064B1
(en)
1994-06-01
AU583723B2
(en)
1989-05-04
US4933434A
(en)
1990-06-12
HK46896A
(en)
1996-03-22
FI93842C
(en)
1995-06-12
SK249587A3
(en)
1999-06-11
DK179787A
(en)
1987-10-09
JPH0742307B2
(en)
1995-05-10
CZ249587A3
(en)
1997-11-12
FI871522A
(en)
1987-10-09
JPS62242697A
(en)
1987-10-23
EP0241022B1
(en)
1993-01-13
DD260280A5
(en)
1988-09-21
EP0241022A2
(en)
1987-10-14
GR3007605T3
(en)
1993-08-31
HUT44046A
(en)
1988-01-28
KR900005279B1
(en)
1990-07-27
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Legal Events
Date
Code
Title
Description
2004-11-11
MK14
Patent ceased section 143(a) (annual fees not paid) or expired
