GB1043840A

GB1043840A – Elution electrophoresis
– Google Patents

GB1043840A – Elution electrophoresis
– Google Patents
Elution electrophoresis

Info

Publication number
GB1043840A

GB1043840A
GB36982/65A
GB3698265A
GB1043840A
GB 1043840 A
GB1043840 A
GB 1043840A
GB 36982/65 A
GB36982/65 A
GB 36982/65A
GB 3698265 A
GB3698265 A
GB 3698265A
GB 1043840 A
GB1043840 A
GB 1043840A
Authority
GB
United Kingdom
Prior art keywords
membrane
components
coated
electrodes
block
Prior art date
1964-09-15
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Expired

Application number
GB36982/65A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Individual

Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
1964-09-15
Filing date
1965-08-27
Publication date
1966-09-28

1965-08-27
Application filed by Individual
filed
Critical
Individual

1966-09-28
Publication of GB1043840A
publication
Critical
patent/GB1043840A/en

Status
Expired
legal-status
Critical
Current

Links

Espacenet

Global Dossier

Discuss

Classifications

G—PHYSICS

G01—MEASURING; TESTING

G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means

G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis

G01N27/416—Systems

G01N27/447—Systems using electrophoresis

G01N27/44704—Details; Accessories

G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones

G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means

Abstract

Electro-phoretically deposited components formed on a membrane from a complex substance, are individually separated into pure components by immersing the membrane in a buffer solution and eluting the components electro phoretically which are drawn off into individual collecting zones. The coated membrane 10 of accylamide gel is first made by depositing the components from the complex mixture by applying the electric field across the face of the membrane, whereby the individual components are deposited in parallel zones or “streaks”. The coated membrane is mounted in a cell 14, Fig. 1. with electrodes 18, 20, the face of the membrane being pressed against a block 22 honeycombed with channels 24 Figs 1, 5, having vertical drain grooves 26. Sponge blocks 32, 34 press the membrane 10, and a second membrane 30 against the block 22. The eluted components are drawn off from their locations in the coated membrane and drain off downwards into individual pots 28. The barrier membrane 30 prevents components from passing through, and drain grooves facing this membrane (similar to grooves 26) provide some convection flow of the buffer eluant. In modifications Fig. 7 (not shown), the pressure sponge blocks 32, 34 are replaced by bags of cellulose material which enclose the electrodes and press against the grid block 22. The buffer eluant is pumped continuously so that anolyte and catholyte are mixed neutralizing any pH change. In Fig. 9, (not shown), the pressure exerted on the coated diaphragm 10 is sufficient to cause the gel body to be forced into the channels 24. The electrodes are frames of insulating material into the edges of which perforated or wire mesh stainless steel is embedded.

GB36982/65A
1964-09-15
1965-08-27
Elution electrophoresis

Expired

GB1043840A
(en)

Applications Claiming Priority (1)

Application Number
Priority Date
Filing Date
Title

US396664A

US3255100A
(en)

1964-09-15
1964-09-15
Elution electrophoresis

Publications (1)

Publication Number
Publication Date

GB1043840A
true

GB1043840A
(en)

1966-09-28

Family
ID=23568142
Family Applications (1)

Application Number
Title
Priority Date
Filing Date

GB36982/65A
Expired

GB1043840A
(en)

1964-09-15
1965-08-27
Elution electrophoresis

Country Status (2)

Country
Link

US
(1)

US3255100A
(en)

GB
(1)

GB1043840A
(en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party

Publication number
Priority date
Publication date
Assignee
Title

US3498905A
(en)

*

1965-06-18
1970-03-03
Beckman Instruments Inc
Continuous flow electrophoresis apparatus

GB1410188A
(en)

*

1970-03-27
1975-10-15
Wallace R A P
Method of and apparatus for removing concentrated solutions of ionisalbe compounds from aqueous solutions thereof

US3718559A
(en)

*

1970-03-27
1973-02-27
R Wallace
Electrogel process for removing electrolytes and concentrating the same from an aqueous solution

US3984298A
(en)

*

1970-12-28
1976-10-05
Haber Instruments, Incorporated
Electromolecular propulsion in semiconductive media

DE2365284C2
(en)

*

1973-12-31
1975-10-23
Medac Gesellschaft Fuer Klinische Spezialpraeparate Mbh, 2000 Hamburg

Chamber and method for performing the second cross-electrophoresis step

US3988230A
(en)

*

1973-12-31
1976-10-26
Medac Gesellschaft Fur Klinische Spezial Praparate Mbh
Chamber and process for crossed immunoelectro-phoresis

JPS5651295B2
(en)

*

1974-11-15
1981-12-04

US3989612A
(en)

*

1974-12-26
1976-11-02
The Upjohn Company
Elution device for gel electrophoresis

US4094759A
(en)

*

1975-01-03
1978-06-13
Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V.
Method for simultaneous quantitative analysis of several constituents in a sample

US4148703A
(en)

*

1976-02-11
1979-04-10
Morton Weintraub
Method of electrophoretic purification of enzymes and peptides by means of an adjustable, specialized, geometrically located electrode system

US4159933A
(en)

*

1977-03-25
1979-07-03
Instrumentation Specialties Company
Sample concentrator

US4088561A
(en)

*

1977-06-27
1978-05-09
The United States Of America As Represented By The United States Department Of Energy
Apparatus for electrophoresis separation

DE2929478A1
(en)

*

1979-07-20
1981-02-05
Max Planck Gesellschaft

METHOD AND DEVICE FOR CARRYING OUT A ONE AND TWO DIMENSIONAL MICROELECTROPHORESIS

US4541910A
(en)

*

1983-11-07
1985-09-17
Yale University
Method for electroblotting macromolecules from a chromatographic gel

JPS6135345A
(en)

*

1984-07-28
1986-02-19
Tetsuro Asao
Device for recovering migration division or the like from gel

FR2574936B1
(en)

*

1984-12-14
1987-08-07
Biolyon

FLAT ANODE AND DEVICE FOR TRANSVERSE ELECTROPHORESIS CONTAINING THE SAME

US5139637A
(en)

*

1991-03-13
1992-08-18
Macconnell William P
Plasmid purification system and method

US5217593A
(en)

*

1991-03-13
1993-06-08
Macconnell William P
Nucleic acid purification system and method

Family Cites Families (4)

* Cited by examiner, † Cited by third party

Publication number
Priority date
Publication date
Assignee
Title

US2555487A
(en)

*

1948-02-27
1951-06-05
United Shoe Machinery Corp
Chromatographic process and apparatus

US3129158A
(en)

*

1961-01-24
1964-04-14
Raymond Samuel
Process for gel electrophoresis

US3140714A
(en)

*

1962-06-28
1964-07-14
Cordis Corp
Blood separation method

US3118829A
(en)

*

1963-03-28
1964-01-21
Raymond Samuel
Electrophoretic destaining apparatus

1964

1964-09-15
US
US396664A
patent/US3255100A/en
not_active
Expired – Lifetime

1965

1965-08-27
GB
GB36982/65A
patent/GB1043840A/en
not_active
Expired

Also Published As

Publication number
Publication date

US3255100A
(en)

1966-06-07

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