GB1378134A – Process for the purification of carcinoembryonic antigen
– Google Patents
GB1378134A – Process for the purification of carcinoembryonic antigen
– Google Patents
Process for the purification of carcinoembryonic antigen
Info
Publication number
GB1378134A
GB1378134A
GB2261173A
GB2261173A
GB1378134A
GB 1378134 A
GB1378134 A
GB 1378134A
GB 2261173 A
GB2261173 A
GB 2261173A
GB 2261173 A
GB2261173 A
GB 2261173A
GB 1378134 A
GB1378134 A
GB 1378134A
Authority
GB
United Kingdom
Prior art keywords
particle size
microgranular
meg
cellulose
capacity
Prior art date
1972-05-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2261173A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
1972-05-12
Filing date
1973-05-11
Publication date
1974-12-18
1972-05-12
Priority claimed from US252700A
external-priority
patent/US3867363A/en
1973-05-11
Application filed by F Hoffmann La Roche AG
filed
Critical
F Hoffmann La Roche AG
1974-12-18
Publication of GB1378134A
publication
Critical
patent/GB1378134A/en
Status
Expired
legal-status
Critical
Current
Links
Espacenet
Global Dossier
Discuss
Classifications
B—PERFORMING OPERATIONS; TRANSPORTING
B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
B01J47/00—Ion-exchange processes in general; Apparatus therefor
B01J47/02—Column or bed processes
B01J47/04—Mixed-bed processes
B—PERFORMING OPERATIONS; TRANSPORTING
B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
B01J39/26—Cation exchangers for chromatographic processes
B—PERFORMING OPERATIONS; TRANSPORTING
B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
B01J47/00—Ion-exchange processes in general; Apparatus therefor
B01J47/014—Ion-exchange processes in general; Apparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
G—PHYSICS
G01—MEASURING; TESTING
G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 – G01N31/00
G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
G01N33/57473—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving carcinoembryonic antigen, i.e. CEA
A—HUMAN NECESSITIES
A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
A61K39/00—Medicinal preparations containing antigens or antibodies
Abstract
1378134 Antigen purification F HOFFMANN-LA ROCHE & CO AG 11 May 1973 [12 May 1972] 22611/73 Heading A5B Human carcino-embryonic antigen is purified by (a) absorbing it on a mixed bed cellulose ion-exchange column comprising (i) microgranular diethylaminoethyl cellulose in the free base form having rod-shaped particles, a capacity of 1.0+0.1 meg/gm, a particle size distribution of 20Á to 60Á and a water regain of 2.3 to 2.8 gm/gm dry exchanger and (ii) a microgranular carboxymethyl cellulose with a capacity of 1.0 0.1 meg/gm, having rod-shaped particles, a particle size distribution of 20Á to 60Á and a water regain of 2.3 to 2.7 gm/gm dry exchanger, (b) eluting the column with an aqueous solution of ammonium acetate and sodium chloride, (c) subjecting the eluate to sequential chromatography first on an agarose gel containing about 6% agarose and having a particle size of 40 to 210Á and then on a hydrophilic water-insoluble cross-linked dextran polymer gel and (d) collecting and concentrating the eluate fractions which have a spectrophotometric absorption peak at 280 mÁ and a molecular weight of 200,000 to 500,000.
GB2261173A
1972-05-12
1973-05-11
Process for the purification of carcinoembryonic antigen
Expired
GB1378134A
(en)
Applications Claiming Priority (1)
Application Number
Priority Date
Filing Date
Title
US252700A
US3867363A
(en)
1970-06-01
1972-05-12
Carcinoembryonic antigens
Publications (1)
Publication Number
Publication Date
GB1378134A
true
GB1378134A
(en)
1974-12-18
Family
ID=22957148
Family Applications (1)
Application Number
Title
Priority Date
Filing Date
GB2261173A
Expired
GB1378134A
(en)
1972-05-12
1973-05-11
Process for the purification of carcinoembryonic antigen
Country Status (15)
Country
Link
JP
(1)
JPS5912990B2
(en)
AU
(1)
AU468779B2
(en)
BE
(1)
BE799405A
(en)
CA
(1)
CA948991A
(en)
CH
(1)
CH583036A5
(en)
DD
(1)
DD107293A5
(en)
DE
(1)
DE2323955A1
(en)
DK
(1)
DK133559B
(en)
FR
(1)
FR2184753B1
(en)
GB
(1)
GB1378134A
(en)
IL
(1)
IL42119A
(en)
IT
(1)
IT998101B
(en)
NL
(1)
NL7306346A
(en)
SE
(1)
SE403912B
(en)
ZA
(1)
ZA732770B
(en)
Families Citing this family (3)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
US4146603A
(en)
*
1977-02-18
1979-03-27
Research Corporation
Tumor specific glycoproteins and method for detecting tumorigenic cancers
CA1225930A
(en)
*
1982-06-07
1987-08-25
Otto A. Gansow
Metal chelate conjugated monoclonal antibodies
JPS6076395A
(en)
*
1983-10-03
1985-04-30
Dainippon Screen Mfg Co Ltd
Eluting device for printing plate
Family Cites Families (1)
* Cited by examiner, † Cited by third party
Publication number
Priority date
Publication date
Assignee
Title
US3697638A
(en)
*
1970-06-01
1972-10-10
Hoffmann La Roche
Antigens
1973
1973-04-11
CH
CH519673A
patent/CH583036A5/xx
not_active
IP Right Cessation
1973-04-20
IT
IT23343/73A
patent/IT998101B/en
active
1973-04-24
ZA
ZA732770A
patent/ZA732770B/en
unknown
1973-04-25
CA
CA169,501A
patent/CA948991A/en
not_active
Expired
1973-04-27
IL
IL42119A
patent/IL42119A/en
unknown
1973-04-30
AU
AU54989/73A
patent/AU468779B2/en
not_active
Expired
1973-05-07
DK
DK249773AA
patent/DK133559B/en
not_active
IP Right Cessation
1973-05-07
NL
NL7306346A
patent/NL7306346A/xx
unknown
1973-05-10
JP
JP48051263A
patent/JPS5912990B2/en
not_active
Expired
1973-05-10
DD
DD170753A
patent/DD107293A5/xx
unknown
1973-05-11
DE
DE2323955A
patent/DE2323955A1/en
active
Pending
1973-05-11
BE
BE131003A
patent/BE799405A/en
not_active
IP Right Cessation
1973-05-11
GB
GB2261173A
patent/GB1378134A/en
not_active
Expired
1973-05-11
SE
SE7306698-7A
patent/SE403912B/en
unknown
1973-05-11
FR
FR7317188A
patent/FR2184753B1/fr
not_active
Expired
Also Published As
Publication number
Publication date
IL42119A0
(en)
1973-06-29
AU468779B2
(en)
1976-01-22
NL7306346A
(en)
1973-11-14
DD107293A5
(en)
1974-07-20
DE2323955A1
(en)
1973-11-22
ZA732770B
(en)
1974-04-24
AU5498973A
(en)
1974-10-31
SE403912B
(en)
1978-09-11
JPS4947521A
(en)
1974-05-08
DK133559C
(en)
1977-02-21
IL42119A
(en)
1976-08-31
CA948991A
(en)
1974-06-11
IT998101B
(en)
1976-01-20
JPS5912990B2
(en)
1984-03-27
FR2184753B1
(en)
1977-11-04
FR2184753A1
(en)
1973-12-28
DK133559B
(en)
1976-06-08
CH583036A5
(en)
1976-12-31
BE799405A
(en)
1973-11-12
Similar Documents
Publication
Publication Date
Title
GB1356911A
(en)
1974-06-19
Purification and fractionation of carcinoembryonic antigen
US4314935A
(en)
1982-02-09
Process for recovering interferon
US3002823A
(en)
1961-10-03
Process of separating materials having different molecular weights and dimensions
FR2668164B1
(en)
1995-04-14
US4359389A
(en)
1982-11-16
Method for the purification of interferon
US4440675A
(en)
1984-04-03
Human immune interferon
GB1378134A
(en)
1974-12-18
Process for the purification of carcinoembryonic antigen
Cifonelli et al.
1957
The purification of hyaluronic acid by the use of charcoal
Sølling et al.
1973
A rapid method for the purification of glycogen synthetase I and D utilising Concanavalin A bound to agarose
GB1286014A
(en)
1972-08-16
Anti-tumour substance and process for producing the same
GB941019A
(en)
1963-11-06
Improvements in and relating to the stability of anti-haemophilic globulin (factor viii)
GB1062434A
(en)
1967-03-22
Process for isolating -antitrypsin
US3634196A
(en)
1972-01-11
Process for the separation of pyrogens from crude preparations of l-asparaginase
Petrovic et al.
1974
A new general method for separation of nucleic acids
Jones et al.
1961
Nucleotides, amino acids and peptides in perchloric acid extracts of Chlamydomonas moewusii
US3651231A
(en)
1972-03-21
Process for preparing lactogenic factor
Phillips et al.
1963
Gel-filtration of the α-Toxin of Clostridium oedematiens Type B
Blandamer et al.
1963
Dextran-gel Filtration in the Purification of a Crustacean α-Amylase
GB1295376A
(en)
1972-11-08
GB1494230A
(en)
1977-12-07
Process for the purification of alkali metal or ammonium insulin
Stancek et al.
1970
Comparison of in vitro and in vivo labeling of virus-induced L-cell interferon
Lerner et al.
1968
Chromatographic separation of biologically important phosphate esters
GB1425918A
(en)
1976-02-25
Process for enzyme purification
GB1494229A
(en)
1977-12-07
Process for the purification of alkali metal or ammonium insulin
ROCHER et al.
1986
Care was taken to prevent exposure of all the solutions containing perphena-zine to light. After coupling, the mixture was vacuum filtered and successively washed with 500 ml dioxane, 750 ml acetone, 500 ml ethanol, 3 1 deionized water and 1 1 of 10 mM Tris-HCl (pH 7.0) containing 0.02% sodium azide, and was kept in this buffer at 4 C until used.
Legal Events
Date
Code
Title
Description
1975-04-30
PS
Patent sealed [section 19, patents act 1949]
1986-12-31
PCNP
Patent ceased through non-payment of renewal fee
