GB1566568A - Creation of Inventions and Patents with Artificial Intelligence

GB1566568A – Device for making a culture of co2 requiring organisms
– Google Patents

GB1566568A – Device for making a culture of co2 requiring organisms
– Google Patents
Device for making a culture of co2 requiring organisms

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Publication number
GB1566568A

GB1566568A
GB43329/76A
GB4332976A
GB1566568A
GB 1566568 A
GB1566568 A
GB 1566568A
GB 43329/76 A
GB43329/76 A
GB 43329/76A
GB 4332976 A
GB4332976 A
GB 4332976A
GB 1566568 A
GB1566568 A
GB 1566568A
Authority
GB
United Kingdom
Prior art keywords
tablet
container
elongate member
packet
culture medium
Prior art date
1975-10-20
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Expired

Application number
GB43329/76A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

GlaxoSmithKline LLC

Original Assignee
SmithKline Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
1975-10-20
Filing date
1976-10-19
Publication date
1980-05-08

1976-10-19
Application filed by SmithKline Corp
filed
Critical
SmithKline Corp

1980-05-08
Publication of GB1566568A
publication
Critical
patent/GB1566568A/en

Status
Expired
legal-status
Critical
Current

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Classifications

C—CHEMISTRY; METALLURGY

C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING

C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS

C12M23/00—Constructional details, e.g. recesses, hinges

C12M23/02—Form or structure of the vessel

C12M23/08—Flask, bottle or test tube

C—CHEMISTRY; METALLURGY

C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING

C12M23/00—Constructional details, e.g. recesses, hinges

C12M23/22—Transparent or translucent parts

C—CHEMISTRY; METALLURGY

C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING

C12M23/00—Constructional details, e.g. recesses, hinges

C12M23/34—Internal compartments or partitions

Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC

Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S435/00—Chemistry: molecular biology and microbiology

Y10S435/801—Anerobic cultivation

Description

PATENT SPECIFICATION
( 21) Application No 43329/76 ( 22) Filed 19 Oct 1976 ( 31) Convention Application No 623936 ( 32) ( 33) ( 44) ( 51) Filed 20 Oct 1975 in United States of America (US)
Complete Specification published 8 May 1980
INT CL 3 C 12 M 1/00 ( 52) Index at acceptance C 6 F CB CIA 1 IB 2 ( 72) Inventors BETTY ANNE BOWIE and JOSEPH FRANK PAGANO ( 54) DEVICE FOR MAKING A CULTURE OF CO 2 REQUIRING ORGANISMS ( 71) We, SMITHKLINE CORPORATION, of 1500 Spring Garden Street, City of Philadelphia, Commonwealth of Pennsylvania 19101, United States of America, a corporation organized under the laws of the Commonwealth of Pennsylvania, one of the United States of America, do hereby declare the invention, for which we pray, that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement:-
This invention relates to devices for culturing CO 2-requiring micro-organisms.
It is known to culture Neisseria gonorrhoeae and other CO 2-requiring organisms in a culture medium retained on an elongate member which is removably positioned in a closed container such as is shown in U S Patent Specification No.
3,616,265 It is known to the art to employ a carbon dioxide (CO 2) tablet usually containing sodium bicarbonate and an organic acid, for example citric acid, in the bottom of the container to provide carbon dioxide for culturing the organism In the presence of moisture, the tablet releases CO 2 Considerable difficulty has been experienced due to the fact that the amount of moisture available to the tablet varies greatly Frequently, excessive moisture condenses in the container, forming a pool of water surrounding the tablet which causes a rapid evolution of carbon dioxide within minutes producing a higher than usual pressure and a greater than usual leakage through the closure of the container, thereby reducing the length of time that carbon dioxide is present in the container to an unsatisfactory time period.
Devices for culturing micro-organisms are also described in British Patent Specification 1,246,070 and in our copending British Patent Application 13892/76 (Serial No 1548605).
According to the present invention there is provided a device for culturing a CO 2requiring micro-organism, the device comprising an elongate member for supporting the organisms to be cultured, an open ended container for the elongate member and for receiving a culture medium for the organism, a seal for sealing the open end of the container, and securing means for securing a CO 2 tablet to the elongate member between the culture medium and the open end of the container with the tablet exposed to the atmosphere within the container.
Devices of the present invention prevent the tablet from being exposed to an accumulation of water by holding the CO 2 tablet above the culture medium This results in a substantially even release of carbon dioxide due to the interaction of moisture in the air within the container and the tablet, and it can ensure the presence of carbon dioxide within the container for a period of time sufficient to carry out a desired culturing, for example two days.
Prior art devices and devices in accordance with the invention for culturing a CO 2-requiring organism will now be described, by way of example, with reference to the accompanying diagrammatic drawings in which:Figure 1 is a side elevation of a single chamber culture device of the prior art;
Figure 2 is a front elevation of the elongate culture medium-supporting member of the device of Figure 1; Figure 3 is a side elevation of a two chamber culture device of the prior art,
Figure 4 is a front elevation of the elongate culture medium-supporting member of the device of Figure 3; Figure 5 is a side elevation of a single chamber culture device in accordance with the invention; Figure 6 is a front elevation of the elongate culture medium-supporting member of the device of Figure 5; ( 11) 1 566 568 1,566,568 Figure 7 is a plan view of a carbon dioxide tablet packet; Figure 8 is a side view of the packet of Figure 7 showing it torn to expose the tablet thereof to the atmosphere; Figure 9 is a side elevation of a double chamber culture device in accordance with the invention; and Figure 10 is a front elevation of the elongate culture medium-supporting member of the device of Figure 9.
There is shown in Figure 1 a culture device 1 known to the prior art The device
1 has a transparent container 2, for example, of, glass or a plastics An elongate member 4 has an inner end 5 and a recessed portion 6 in each opposed substantially flat face 8.
Each recessed portion 6 is intermediate the ends of the elongate member 4 and has multiple small protrusions 10 to facilitate the retention of a body 12 of a solid culture medium in the recessed portions The elongate member 4 has a handle 16 and a plug portion 18 which is adapted to be sealingly engaged within cylindrical member 20 which has a plug portion 22 received within the open end 24 of container 2 Such a container is disclosed in detail in United States Patent Specification
No 3,616,265 When organisms are being cultured, it is known to introduce into the bottom of container 2 a carbon dioxide tablet 26.
It is also known to employ a double chamber culture device such as that shown at 32 in Figure 3 The device 32 has a transparent container 34 containing an elongate member 36 which supports a culture medium 38 in recesses indicated at 40, 40 in opposite faces 42, 42 of the elongate member 36 and having protrusions 43 Elongate member 36 extends through a ring seal 46 which together with the elongate member 36 separates container 34 into two separate compartments 50 and 52.
Chamber 52 contains a liquid culture medium 54 Elongate member 36 has a handle 60 and a plug portion 62 which sealingly engages a cylindrical member 64 which, in turn, has a plug portion 66 which sealingly engages the open end 68 of container 34 Culture devices such as the device 32 have not been employed where it is desired to have carbon dioxide in the upper chamber 50, due to the difficulty of providing a carbon dioxide tablet in chamber 50.
A culture device 70 in accordance with the invention has a transparent container 72 containing an elongate member 74 with recesses 76, 76 in opposite faces 78, 78 containing culture medium 82 and protrusions 83 Elongate member 74 has a handle 86 and a plug portion 88 which sealingly engages a cylindrical member 90 which, in turn, has a plug portion 92 mounted in the open end 94 of container 72.
As thus described, the device 70 is the same as the device 1 Elongate member 74 has a slot 100 in which there is secured a packet 102 containing a carbon dioxide tablet 104.
Packet 102 can be, for example, opposed sheets 106 and 108 of a plastics such as Cellophane (registered Trade Mark), polyethylene or polypropylene, or a metal foil such as aluminum or tinfoil heat sealed at its edges 110 to be air tight or a combination thereof Such packets are well known to the art and hence need not be described in greater detail CO 2 tablets are also well known Typically the CO 2 tablet 104 will contain a carbonate, for example sodium bicarbonate, and a water-soluble organic acid, for example citric acid or tartaric acid CO 2 tablets are available commercially, for example, “TAB-CO 2 ” sold by Ames Company Inc, 1127 Myrtle Street, Elkhart, Indiana, U S A As shown in Figure 7, the packet is activated by punching a pinhole 112 in sheet 106 to provide for the admission of air and moisture within packet 102 to the CO 2 tablet 104 and the exit of the formed carbon dioxide.
As best seen in Figure 6, slot 100 is sized to provide for a wedge fit of the packet 102.
In operation, the sealed packet 102 is punctured to form a pinhole 112, and then the packet is securely wedged in the slot A specimen suspected of including a particular micro-organism is brought into contact with the culture medium 82, and the elongate member 74 is placed in the container 72 If a somewhat faster release of CO 2 is desired, the packet 102 can be provided with a tear 116 adjacent the tablet 104, as illustrated in Figure 8.
Referring now to Figure 9, a two compartment culture device 120 has a transplant container 122 containing an elongate member 124 with recesses 126 in opposite faces 128, 128 containing culture medium 132 and protrusions 129 Elongate member 124 has a handle 136 and a plug portion 138 which sealingly engages a cylindrical member 140 which, in turn, has a plug portion 142 which engages the open end 144 of the container 122 Elongate member 124 passes downwardly through a seal ring 150 which separates the container 122 into an upper compartment 152 and a lower compartment 154 containing a liquid culture medium 156 As thus described the culture device 120 is the same as the culture device 32 shown in Figure 3.
Elongate member 124 has a slot 160 therein which is sized to receive and hold by a wedged fit a packet 102 containing a CO 2 tablet 104.
In operation, the elongate member 124 is LL 1,566,568 removed from the container 122 while holding the container in a vertical position to retain the liquid culture medium 156 in compartment 154 The container is placed in a holder to maintain it vertical, and the packet 102 is pierced to provide an opening 112 and the packet 102 is then securely wedged in slot 160 The selected specimens are then placed on the lower end of the elongate member 124 for introduction into the liquid culture medium 156 and on the culture medium 126 for culturing in the compartment 152 in the presence of carbon dioxide Elongate member 124 is then replaced in the container 122 After a suitable period of incubation observations can be made through the transparent walls of the container 122.
A typical solid culture medium for culturing Neisseria gonorrhoeae is described by Martin, J E, Jr and Lester, A in HSMHA Health Reports, Volume 86, pages 30-33, 1971.
An example of a CO 2 tablet is a tablet made with a 3/16 ‘ punch and having the following composition:
Citric Acid (U S P.
anhydrous granular) Sodium Bicarbonate (U S P) Terra Alba (U S P) Magnesium Stearate (U.S P) mg.
21.320 28.000 8.650 0.073 Carbon dioxide within the culturing container is desirable when culturing certain micro-organisms contained in body liquids Typical of such micro-organisms are Neisseria gonorrhoeae and Streptococci.

Claims (6)

WHAT WE CLAIM IS:-

1 A device for culturing a CO 2-requiring micro-organism, the device comprising an elongate member for supporting the organisms to be cultured, an open-ended container for the elongate member and for receiving a culture medium for the organism, a seal for sealing the open end of the container, and securing means for securing a CO 2 tablet to the elongate member between the culture medium and the open end of the container with the tablet exposed to the atmosphere within the container.

2 A device in accordance with claim 1, in, which the securing means comprises an aperture in the elongate member for receiving the CO 2 tablet in a packet.

3 A device in accordance with claim I or claim 2, which includes the CO 2 tablet, the tablet containing sodium bicarbonate and citric acid.

4 A device in accordance with any one of claims I to 3, which includes the CO 2 tablet which is in a packet having a pinhole opening adjacent the tablet.

A device in accordance with any one of claims 1 to 3, which includes the CO 2 tablet which is in a packet having a tear adjacent the tablet.

6 A device for making a culture of a CO 2requiring micro-organism, the device being substantially as herein described with reference to Figures 5 to 8 or Figures 7 to 10 of the accompanying drawings.
G H HARGREAVES, Chartered Patent Agent.
Printed for Her Majesty’s Stationery Office, by the Courier Press, Leamington Spa, 1980 Published by The Patent Office, 25 Southampton Buildings London WC 2 A l AY, from which copies may be obtained.

GB43329/76A
1975-10-20
1976-10-19
Device for making a culture of co2 requiring organisms

Expired

GB1566568A
(en)

Applications Claiming Priority (1)

Application Number
Priority Date
Filing Date
Title

US05/623,936

US3961696A
(en)

1975-10-20
1975-10-20
Device for making a culture of CO2 requiring organisms

Publications (1)

Publication Number
Publication Date

GB1566568A
true

GB1566568A
(en)

1980-05-08

Family
ID=24499955
Family Applications (1)

Application Number
Title
Priority Date
Filing Date

GB43329/76A
Expired

GB1566568A
(en)

1975-10-20
1976-10-19
Device for making a culture of co2 requiring organisms

Country Status (19)

Country
Link

US
(1)

US3961696A
(en)

JP
(1)

JPS5842757B2
(en)

AT
(1)

AT352059B
(en)

AU
(1)

AU506019B2
(en)

BE
(1)

BE846955A
(en)

BR
(1)

BR7607015A
(en)

CA
(1)

CA1071128A
(en)

CH
(1)

CH624429A5
(en)

DE
(1)

DE2645932A1
(en)

ES
(1)

ES242123Y
(en)

FI
(1)

FI55218C
(en)

FR
(1)

FR2328769A1
(en)

GB
(1)

GB1566568A
(en)

IT
(1)

IT1067026B
(en)

LU
(1)

LU76023A1
(en)

MX
(1)

MX144207A
(en)

NL
(1)

NL7611587A
(en)

NO
(1)

NO147882C
(en)

SE
(1)

SE7610800L
(en)

Cited By (1)

* Cited by examiner, † Cited by third party

Publication number
Priority date
Publication date
Assignee
Title

GB2141136A
(en)

1983-06-08
1984-12-12
Basildon Moulding Company Limi
Dip slide

Families Citing this family (5)

* Cited by examiner, † Cited by third party

Publication number
Priority date
Publication date
Assignee
Title

US4082614A
(en)

1975-12-10
1978-04-04
Bio-Syn, Inc.
Means for conveying pathological specimens safely to places of identification

US4014748A
(en)

1975-12-22
1977-03-29
Marion Laboratories, Inc.
Anaerobic culture collecting and transporting apparatus

US4347222A
(en)

1981-04-21
1982-08-31
Miles Laboratories, Inc.
Gas generating apparatus for anaerobic atmosphere

FI834276A
(en)

1982-12-10
1984-06-11
Hoffmann La Roche

BEHAOLLARE.

GB8820594D0
(en)

1988-08-31
1988-09-28
Corbett R J
Apparatus & method for determining presence of microorganisms

Family Cites Families (6)

* Cited by examiner, † Cited by third party

Publication number
Priority date
Publication date
Assignee
Title

US3616263A
(en)

1969-06-16
1971-10-26
Univ Tennessee Res Corp
Gas cap for culture tubes

US3616265A
(en)

1969-08-07
1971-10-26
Smith Kline French Lab
Device for making a culture of microorganisms

US3817839A
(en)

1971-03-29
1974-06-18
D Warren
Bi-media dip plate

US3862013A
(en)

1973-05-24
1975-01-21
Smithkline Corp
Device for making a culture of micro-organisms

US3888741A
(en)

1974-04-25
1975-06-10
Miles Lab
Culturing means and test kit

US3891509A
(en)

1974-05-06
1975-06-24
Clinical Convenience Products
CO{HD 2 {B generating tablet for culture devices

1975

1975-10-20
US
US05/623,936
patent/US3961696A/en
not_active
Expired – Lifetime

1976

1976-06-29
JP
JP51077670A
patent/JPS5842757B2/en
not_active
Expired

1976-09-29
SE
SE7610800A
patent/SE7610800L/en
not_active
Application Discontinuation

1976-10-05
BE
BE171251A
patent/BE846955A/en
not_active
IP Right Cessation

1976-10-12
IT
IT28243/76A
patent/IT1067026B/en
active

1976-10-12
DE
DE19762645932
patent/DE2645932A1/en
active
Granted

1976-10-12
AU
AU18585/76A
patent/AU506019B2/en
not_active
Expired

1976-10-14
CH
CH1303076A
patent/CH624429A5/de
not_active
IP Right Cessation

1976-10-15
CA
CA263,438A
patent/CA1071128A/en
not_active
Expired

1976-10-18
AT
AT772776A
patent/AT352059B/en
not_active
IP Right Cessation

1976-10-18
LU
LU76023A
patent/LU76023A1/xx
unknown

1976-10-18
MX
MX166692A
patent/MX144207A/en
unknown

1976-10-18
FI
FI762970A
patent/FI55218C/en
not_active
IP Right Cessation

1976-10-19
FR
FR7631416A
patent/FR2328769A1/en
active
Granted

1976-10-19
GB
GB43329/76A
patent/GB1566568A/en
not_active
Expired

1976-10-19
ES
ES1976242123U
patent/ES242123Y/en
not_active
Expired

1976-10-19
NO
NO763564A
patent/NO147882C/en
unknown

1976-10-20
NL
NL7611587A
patent/NL7611587A/en
not_active
Application Discontinuation

1976-10-20
BR
BR7607015A
patent/BR7607015A/en
unknown

Cited By (1)

* Cited by examiner, † Cited by third party

Publication number
Priority date
Publication date
Assignee
Title

GB2141136A
(en)

1983-06-08
1984-12-12
Basildon Moulding Company Limi
Dip slide

Also Published As

Publication number
Publication date

NO147882C
(en)

1983-06-29

IT1067026B
(en)

1985-03-12

MX144207A
(en)

1981-09-10

CH624429A5
(en)

1981-07-31

FR2328769B1
(en)

1979-10-12

LU76023A1
(en)

1977-05-16

DE2645932C2
(en)

1987-04-23

AU1858576A
(en)

1978-04-20

ES242123Y
(en)

1979-11-16

BR7607015A
(en)

1977-09-06

FI55218C
(en)

1979-06-11

CA1071128A
(en)

1980-02-05

AU506019B2
(en)

1979-12-13

NO763564L
(en)

1977-04-21

AT352059B
(en)

1979-08-27

JPS5251087A
(en)

1977-04-23

SE7610800L
(en)

1977-04-21

US3961696A
(en)

1976-06-08

NO147882B
(en)

1983-03-21

ES242123U
(en)

1979-06-01

JPS5842757B2
(en)

1983-09-21

ATA772776A
(en)

1979-02-15

FR2328769A1
(en)

1977-05-20

DE2645932A1
(en)

1977-04-21

BE846955A
(en)

1977-04-05

NL7611587A
(en)

1977-04-22

FI762970A
(en)

1977-04-21

FI55218B
(en)

1979-02-28

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